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US 2003/0153741 A1Published application
Methods for separating oligonucleotides
OwnerMarek Kwiatkowskiinventor
Life of this patent
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Abstract
Methods for separating oligonucleotides are described. The oligonucleotides to be separated include separation tags attached to each end of the oligonucleotides.
Claims33
A method for separating oligonucleotides, said method comprising:
- a) providing a plurality of oligonucleotides, said plurality of oligonucleotides comprising at least one bifunctional oligonucleotide and at least one non-bifunctional oligonucleotide, wherein each said at least one bifunctional oligonucleotide comprises a first separation tag attached to a first end of said at least one bifunctional oligonucleotide and a second separation tag attached to a second end of said at least one bifunctional oligonucleotide, and wherein cleavage of said first or said second separation tags yields an oligonucleotide having a 3′hydroxyl moiety;
- b) contacting said plurality of oligonucleotides with a separation medium under conditions effective for adhering said at least one bifunctional oligonucleotide to said separation medium;
- and
- c) selectively eluting at least one non-bifunctional oligonucleotide.
1 more element
A method for separating oligonucleotides, said method comprising:
- a) providing a plurality of oligonucleotides, wherein said plurality of oligonucleotides comprises at least one bifunctional oligonucleotide and at least one non-bifunctional oligonucleotide, and wherein each said at least one bifunctional oligonucleotide comprises a first separation tag attached to a first end of said at least one bifunctional oligonucleotide and a second separation tag attached to a second end of said at least one bifunctional oligonucleotide, and wherein cleavage of said first or said second separation tags yields an oligonucleotide having a 3′ hydroxyl moiety;
- b) contacting said plurality of oligonucleotides with a separation medium under conditions effective for adhering said at least one bifunctional oligonucleotide to said separation medium;
- c) eluting non-bifunctional oligonucleotides lacking said first separation tag without eluting said bifunctional oligonucleotides;
- d) cleaving said first separation tag from the oligonucleotides retained on the separation medium;
- and
- e) eluting non-bifunctional oligonucleotides lacking said second separation function.
3 more elements
A composition comprising:
- a) a plurality of oligonucleotides, each said oligonucleotide comprising a first separation tag attached to a first end of said oligonucleotide and a second separation tag attached to a second end of said oligonucleotide, wherein cleavage of said first or said separation tags yields an oligonucleotide having a 3′ hydroxyl moiety;
- and
- b) a separation medium, said plurality of oligonucleotides adhering to said separation medium.
Dependencies are inferred from the claim text.