Protein purification
AssigneeGenentech, Inc.
InventorsTimothy N. Breece, Robert L. Fahrner, Jeffrey R. Gorrell, Kathlyn Pham Lazzareschi, Philip M. Lester, David Peng
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Abstract
A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
Claims20
A method for purifying a protein, which comprises a CH2/CH2 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising detergent and salt;
- and
- (c) recovering the protein from the solid phase.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising a buffer at a concentration of greater than about 0.8M;
- and
- (c) recovering the protein from the solid phase.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising salt and solvent;
- and
- (c) recovering the protein from the solid phase.
A method for purifying a protein, which comprises a CH2/CH3 region, from a contaminated solution thereof by Protein A chromatography comprising:
- (a) adsorbing the protein to Protein A immobilized on a solid phase;
- (b) removing contaminants by washing the solid phase with a composition comprising salt and a polymer;
- and
- (c) recovering the protein from the solid phase.
Dependencies are inferred from the claim text.