US 2003/0153522 A1 · Rat pituitary tumor transforming gene (PTTG) carboxy-terminal peptides and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Abstract

Disclosed is a method of inhibiting neoplastic cellular proliferation and/or transformation of mammalian cells, including cells of human origin, in vitro or in vivo. The inventive method involves the use of a composition containing a pituitary tumor transforming gene carboxy-terminal peptide (PTTG-C), which can be comprised in a chimeric protein, which has the ability to regulate endogenous pituitary tumor transforming gene (PTTG) expression and/or function in a dominant negative manner. Kits comprising the inventive compositions are also disclosed for the treatment of neoplastic cellular proliferation in vitro or in vivo. Isolated PTTG-C peptides and PTTG-C-containing chimeric proteins are described.

Claims26

Dependent claims

A method of inhibiting neoplastic cellular proliferation and/or transformation of a cell of mammalian origin comprising:

delivering to a mammalian cell a composition comprising a PTTG-C peptide selected from the group consisting of
(A) a PTTG peptide having an amino acid sequence consisting of (SEQ ID NO:
16);
and
(B) peptide fragments of (SEQ ID NO. 16) that comprise at least 15 contiguous amino acid residues that include a proline-rich region, and that function to downregulate endogenous PTTG expression and/or PTTG function,
said expression vector being complexed with a cellular uptake-enhancing agent, in an amount and under conditions sufficient to enter the cell, such that the PTTG-C peptide is expressed in the cell, whereby neoplastic cellular proliferation and/or transformation of the cell is inhibited.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/261,787Filed 30 Sep 2002

Priority

21 Nov 1996

Published

14 Aug 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

A01K 67/027
Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals: new or modified breeds of vertebrates
A61K 48/00
Medicinal preparations containing genetic material which is inserted into cells of the
C07K 14/47
From vertebrates: from mammals
C07K 14/82
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: translation products from oncogenes

Family

Application60/031,338Us provisional application · filed 21 Nov 1996

Application60/065,825Us provisional application · filed 14 Nov 1997

Application08/894,251Continuation in part · filed 23 Jul 1999

Application08/894,251A 371 of international · filed 23 Jul 1999

Application09/569,956Division · filed 12 May 2000

Application09/569,956Continuation in part · filed 12 May 2000

Application10/261,787Division · filed 30 Sep 2002

US 6,455,305 B1Related patent

US 6,894,031 B1Related patent

US 2003/0153522 A1This patent

From the US filing history. Foreign family members aren’t listed.

Rat pituitary tumor transforming gene (PTTG) carboxy-terminal peptides and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Life of this patent

Rat pituitary tumor transforming gene (PTTG) carboxy-terminal peptides and methods of use thereof to inhibit neoplastic cellular proliferation and/or transformation

Life of this patent

Abstract

Claims26