Methods and compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin
OwnerRobert M. Winslow, Kim D. Vandegriftinventors
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Abstract
The present invention relates to blood products, and more particularly to compositions comprising a modified oxygenated hemoglobin having a high affinity for oxygen and methods for making such compositions. Such compositions according to the present invention have better stability to autooxidation and superior oxygen carrying characteristics.
Claims11
A blood substitute product comprising surface-modified oxygenated hemoglobin, wherein the surface-modified oxygenated hemoglobin has a P50 less than native stroma-free hemoglobin from the same animal source when measured under the same conditions.
A blood substitute product stable to autooxidation at 24° C. comprising a PEG-hemoglobin conjugate, wherein the methemoglobin/total hemoglobin ratio is less than 0.10 and the PEG-hemoglobin conjugate has a P50 less than 10 torr.
A method of making a blood substitute product comprising the steps of:
- a) preparing hemoglobin by oxygenating the hemoglobin having a methemoglobin/total hemoglobin ratio less than 0.10;
- b) covalently attaching at least one polyalkylene oxide to the hemoglobin to form surface-modified oxygenated hemoglobin having a P50 less than 10 torr;
- and
- c) suspending the surface-modified oxygenated hemoglobin in an aqueous diluent.
Dependencies are inferred from the claim text.