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AssigneeBoard of Trustees of Michigan State University
InventorsEvangelyn C. Alocilja, Zarini Muhammad-Tahir
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A multi-array membrane strip biosensor device (10, 20) using a fluid mobile conductive polymer as reporter is described. The biosensor device (20) is designed to detect multiple analytes at low concentrations in near real-time with an electronic data collection system. The biosensor device can be small. The device can be used to detect pathogens, proteins, and other biological materials of interest in food, water, and environmental samples. The device can also be used for on-site diagnosis and against potential bioterrorism. Potential users include food processing plants, meat packaging facilities, fruit and vegetable packers, restaurants, food and water safety inspectors, food wholesalers and retailers, farms, homes, medical profession, import border crossing personnel, and the police force, military, space habitation and national security.
1- A biosensor device which comprises:
2- The device of claim 1 wherein the device further comprises a third zone adjacent to the first zone into which the fluid is absorbed after passing through the first defined area of the first zone.
3- The device of any one of claims 1 or 2 wherein the first defined area has a dimension between the electrodes of 1.0 mm or less.
4- A method for detecting an analyte in a fluid sample which comprises:
5- The method of claim 4 wherein the device further comprises a third zone adjacent to the first zone into which the fluid is absorbed after passing through the first defined area of the first zone.
6- The method of claims 4 or 5 wherein the first defined area has a dimension between the electrodes of 1.0 mm or less between which the conductivity is measured.
7- A system for detecting an analyte in a fluid sample which comprises:
8- A biosensor device which comprises:
9- The device of claim 8 wherein the device further comprises a third zone adjacent to the first zone into which the fluid is absorbed after passing through the first defined area of the first zone.
10- The device of any one of claims 8 or 9 wherein the first defined area has a dimension between the electrodes of 1.0 mm or less.
11- A method for detecting an antigen in a fluid sample which comprises:
12- The method of claim 11 wherein the device further comprises a third zone adjacent to the first zone into which the fluid is absorbed after passing through the first defined area of the first zone.
13- The method of claims 11 or 12 wherein the first defined area has a dimension between the electrodes of 1.0 mm or less between which the conductivity is measured.
14- A system for detecting an antigen in a fluid sample which comprises:
15- The system of claim 13 wherein the device further comprises a third zone adjacent to the first zone into which the fluid is absorbed after passing through he first defined area of the first zone.
16- The device of claim 1 or 2 wherein a third substrate for applying the fluid containing the analyte which is applied prior to being introduced into the second zone.
17- The method of claims 4 or 5 wherein a pad for applying the fluid containing the analyte which is applied prior to being introduced into the second zone.
18- The system of claim 7 or 8 wherein a pad for applying the fluid containing the analyte is applied prior to being introduced into the second zone.
19- The device of claim 8 or 9 wherein a pad for applying the fluid containing the analyte is applied prior to being introduced into the second zone.
20- The method of claim 11 or 12 wherein a pad for applying the fluid containing the analyte is applied prior to being introduced into the second zone.
21- The system of claim 14 or 15 wherein a pad for applying the fluid containing the analyte is applied prior to being introduced into the second zone.
22- The device of claim 1 or 2 wherein there is a multiple array so that multiple analytes can be detected simultaneously from the same sample.
23- The method of claim 4 or 5 wherein there is a multiple array so that multiple analytes are detected simultaneously from the same sample.
24- The device of claim 8 or 9 wherein there is a multiple array so that multiple analytes are detected simultaneously from the same sample.
25- The method of claim 11 or 12 wherein there is a multiple array so that multiple analytes are detected simultaneously from the same sample.
26- The system of claim 14 or 15 wherein there is a multiple array so that multiple analytes can be detected simultaneously from the sample.