Methods for improving conifer embryogenesis
AssigneeGeorgia Tech Research Corporation
InventorGerald S. Pullman
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Abstract
The present invention provides methods for initiating, capturing, maintaining and multiplying embryogenic cultures of coniferous plants. Methods include the use of novel media compositions containing Vitamin B12, Vitamin E, or organic acids including α-ketoglutaric acid, pyruvic acid, or p-aminobenzoic acid to improve the frequency of embryogenic tissue initiation, capture, maintenance and multiplication. The methods are well suited for initiating embryogenic cultures in recalcitrant conifer varieties. The method is also well suited for producing somatic embryos that can be further cultured to produce large numbers of plants. Further, the invention provides novel methods that may be used to enhance somatic embryogenesis in a broad range of species.
Claims38
A method of initiating conifer embryogenic cultures comprising a step of culturing explants using a medium comprising vitamin E.
A method of initiating conifer embryogenic cultures comprising a step of culturing explants using a medium comprising vitamin B12.
A method of initiating conifer embryogenic cultures comprising a step of culturing explants using a medium comprising an organic acid.
A method of growing previously initiated conifer embryogenic tissues, comprising the step of culturing said tissues in a medium comprising vitamin E.
A method of growing previously initiated conifer embryogenic tissues, comprising the step of culturing said tissues in a medium comprising vitamin B12.
A method of growing previously initiated conifer embryogenic tissues, comprising the step of culturing said tissues in a medium comprising an organic acid.
Dependencies are inferred from the claim text.