Method for in vitro production of three-dimensional vital cartilage or bone tissue and use thereof as transplant material
AssigneeCo.don Aktiengesllschaft
InventorsJeanette Libera, Ursula Anderer, Karl-Gerd Fritsch, Olivera Josimovic-Alasevic
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Abstract
The invention relates to a remarkably simple method for the in vitro production of three-dimensional, vital and mechanically stable cartilage or bone tissue and to the use thereof as a transplantation material for treating cartilage or bone defects and degenerative diseases such as rheumatism or arthrosis, and to the use thereof in testing active substances and physical factors. The invention is also directed to the cartilage or bone tissue and therapeutical formulations produced thereby, e.g. injection solutions comprising such tissue.
Claims13
A method for the in vitro production of three-dimensional vital cartilage or bone tissue,
- characterized in that
- cartilage cells, bone cells, or mesenchymal stem cells are obtained from a human or animal organism, and these cells are subjected to stationary culturing in the form of a suspension culture in cell culture vessels with hydrophobic surface and tapering bottom, until a cell aggregate is formed which includes at least 40% by volume of extracellular matrix (ECM) having differentiated cells embedded therein, and which has an outer region wherein cells capable of proliferation and migration are present.
Dependencies are inferred from the claim text.