Expansion of T cells in vitro and expanded T cell populations
OwnerPaul Rogers, Toshifumi Mikayamainventors
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Abstract
Methods for expanding T cells (e.g., Vα24+Vβ11+ human NKT cells ) in vitro by repeated culturing in the presence of an antigen (e.g., glycosphingolipid), antigen presenting cells, and a cell survival factor are provided. A population of NKT cells (Vα24+ζβ11+) can be expanded more than 1 million-fold in 5 weeks and more than 1 billion-fold in 10 weeks.
Claims73
A method for stimulating the proliferation of human T cells in vitro comprising repeatedly culturing donor T cells in the presence of antigen presenting cells, an antigen, a cell survival factor and serum for at least 7 days under conditions stimulating proliferation of the T cells.
A method for stimulating the proliferation of human T cells in vitro comprising repeatedly culturing donor T cells in the presence of antigen presenting cells, an antigen, IL-2, without IL-7 or IL-15, and serum under conditions stimulating proliferation of the T cells.
A method for producing a population of proliferated human T cells, comprising repeatedly culturing donor T cells in the presence of antigen presenting cells, an antigen, a cell survival factor and serum for at least 7 days, thereby producing a population of proliferated human T cells.
A method for producing a population of proliferated human T cells, comprising repeatedly culturing donor T cells in the presence of antigen presenting cells, an antigen, IL-2, without IL-7 or IL-15, and serum, thereby producing a population of proliferated human T cells.
Dependencies are inferred from the claim text.