Oligomer for immobilizing physiological material, and composition for immobilizing physiological material comprising the same

A method for preparing an oligomer, comprising:

• mixing an aminosilane compound represented by the following formula (1) with an alkylsilane oligomeric compound represented by the following formula (2)
• H2N—R2—Si(R1)3 (1)
• 4
• wherein
• R1 is selected from the group consisting of hydroxy and C1-14 alkoxy,
• R2 is selected from the group consisting of C1-14 alkyl groups, C6-20 aromatic groups, ether groups, ester groups, and imine groups,
• R3 is C1-12 alkyl,
• R4 is selected from the group consisting of hydroxy, C1-14 alkoxy, halogen, and combinations thereof, and
• n is an integer ranging from 5 to 30;
• adding water to the mixture to prepare an aqueous solution;
• and
• copolymerizing the aminosilane compound of formula (1) with the alkylsilane oligomeric compound of formula (2) while agitating the aqueous solution to obtain the silane oligomer in hydrate.

A coating composition for an immobilization layer for immobilizing a physiological material comprising a silane oligomer that is prepared by copolymerizing an aminosilane compound represented by the following formula (1) with an alkylsilane oligomeric compound represented by the following formula (2):

• H2N—R2—Si(R1)3 (1)
• 5
• wherein
• R1 is selected from the group consisting of hydroxy and C1-14 alkoxy;
• R2 is selected from the group consisting of C1-14 alkyl groups, C6-20 aromatic groups, ether groups, ester groups, and imine groups;
• R3 is C1-12 alkyl;
• R4 is selected from the group consisting of hydroxy, C1-14 alkoxy, halogen, and combinations thereof;
• and
• n is an integer ranging from 5 to 30.

A method for preparing a substrate for immobilizing a physiological material, comprising:

• coating a composition comprising a silane oligomer on a substrate, wherein the silane oligomer is prepared by copolymerizing an aminosilane compound represented by the following formula (1) with an alkylsilane oligomeric compound represented by the following formula (2):
• H2N—R2—Si(R1)3 (1)
• 6
• wherein
• R1 is selected from the group consisting of hydroxy and C1-14 alkoxy,
• R2 is selected from the group consisting of C1-14 alkyl groups, C6-20 aromatic groups, ether groups, ester groups, and imine groups,
• R3 is C1-12 alkyl,
• R4 is selected from the group consisting of hydroxy, C1-14 alkoxy, halogen, and combinations thereof, and
• n is an integer ranging from 5 to 30;
• and
• heat-treating the coated substrate to prepare the three-dimensional cross-linking structural immobilization layer through condensation of the silane oligomer.