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AssigneeCheil Jedang Corporation
InventorsKap-Soo Noh, Yeong-Chul Kim, Jae-Yong Park, Dai-Chul Kim, Jin-Ho Lee, Seung-Han Ok
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A method for producing L-threonine using a microorganism is provided. In the method, additional one or more copies of each of the phosphoenolpyruvate carboxylase (ppc) gene and the threonine operon are integrated into a particular site of the chromosomal DNA of a microorganism, while its inherent ppc gene and threonine operon remain. Accordingly, two or more ppc genes and threonine operons are included in the chromosomal DNA of the microorganism to thereby enhance the expression of the ppc gene encoding an enzyme to convert phosphoenolpyruvate to a threonine biosynthesis precursor, oxaloacetete, and the genes encoding enzymes involved in the synthetic pathway of threonine from oxaloacetate, including thrA (aspartokinasel-homoserine dehydrogenase), thrB (homoserine kinase), and thrC (threonine synthase), thereby markedly increasing L-threonine productivity.
A method of producing L-threonine using a microorganism, one or more copies of each of the phosphoenolpyruvate carboxylase gene and the threonine operon are additionally integrated into a particular site of the chromosomal DNA of the microorganism, while its inherent phosphoenolpyruvate carboxylase gene and threonine operon remain.
Escherichia coli strain pGmTN-PPC12 of Accession No. KCCM 10236 capable of L-threonine production.
Dependencies are inferred from the claim text.