Cholesterol oxidase
OwnerRikizo Aono, Toru Hamaya +1inventors
InventorsRikizo Aono, Toru Hamaya, Toshiaki Kono
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Abstract
A novel cholesterol oxidase is disclosed. The enzyme according to the present invention has the following properties: acts on cholesterol to convert it to cholest-5-en-3-one; acts 3β-sterols but not on 3α-hydroxysteroids; optimum pH: 5.0-8.5; and stable pH: 4 to 11. This enzyme carries out the oxidation reaction at high velocity at low substrate concentrations, reacts in a broad pH range, is heat resistant, and reacts at high reaction velocity even in the presence of an organic solvent. The enzyme according to the present invention is useful as a reagent for measuring cholesterol concentration, as a composition for the extermination of harmful insects, and as a bleaching agent.
Claims14
Cholesterol oxidase produced by ST-200 strain (FERM BP-6661):
An enzyme having the following properties:
- (1) Function:
- acting on cholesterol to convert it to cholest-5-en-3-one;
- acting on cholest-5-en-3-one to convert it to 6β-perhydroxycholest-4-en-3-one;
- (2) Substrate specificity:
- acts on 3β-sterols and not on 3α-hydroxysteroid;
- (3) Optimum pH:
- 5.0 to 8.5;
- and
- (4) Stable pH:
- 4 to 11.
Dependencies are inferred from the claim text.