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AssigneeSankyo Company, Limited
InventorsMasaaki Goto, Eisuke Tsuda, Shin?apos;ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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A protein which inhibits osteoclast differentiation and/or maturation and a method of production of the protein. The protein is produced by human embryonic lung fibroblasts and has molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis, respectively. The protein can be isolated and purified from culture medium of the said fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity to the protein or a method for determination of the protein concentration using the antibodies.
An osteoclastogenesis inhibitory factor protein comprising the following properties:
A method of producing an osteoclastogenesis inhibitory factor protein comprising the steps of
A protein comprising an amino acid sequence as provided in Seq. ID No. 4.
cDNAs encoding an amino acid sequence as provided in Seq. ID No. 4.
cDNA comprising a nucleotide sequence as provided in Seq. ID No. 6.
cDNAs that hybridize to a cDNA as provided in Seq. ID No. 6 under moderately stringent conditions.
A protein expressed from cDNA encoding an amino acid sequence as provided in Seq. ID No. 4.
A protein having a biological activity to inhibit osteoclast differentiation or maturation, said protein having an amino acid sequence expressed from a cDNA sharing at least about 80% sequence identity with the amino acid sequence provided in Seq. ID No. 4.
A recombinant protein which inhibits osteoclast differentiation or maturation expressed from a cDNA encoding an amino acid sequence as provided in Seq. ID No. 4;
A cDNA comprising a nucleotide sequence as provided in Seq. ID No. 8.
A protein encoded by a cDNA having a nucleotide sequence as provided in Seq. ID No. 8.
cDNAs encoding amino acid sequence as provided in Seq. ID No. 9.
A cDNA comprising a nucleotide sequence as provided in Seq. ID No. 10.
A protein encoded by a cDNA comprising a nucleotide sequence as provided in Seq. ID No. 10.
cDNAs encoding an amino acid sequence as provided in Seq. ID No. 11.
A cDNA comprising a nucleotide sequence as provided in Seq. ID No. 12.
A protein encoded by a cDNA having a nucleotide sequence as provided in Seq. ID No. 12.
cDNAs encoding an amino acid sequence as provided in Seq. ID No. 13.
A cDNA comprising a nucleotide sequence as provided in Seq. ID No. 14.
A protein encoded by a cDNA having a nucleotide sequence as provided in Seq. ID No. 14.
cDNAs encoding an amino acid sequence as provided in Seq. ID No. 15.
Dependencies are inferred from the claim text.