Parathyroid hormone receptor and DNA encoding same
AssigneeThe General Hospital Corporation, a Massachusetts corporation
InventorsGino V. Segre, Henry M. Kronenberg, Abdul-Badi Abou-Samra, Harald Juppner, John T. Potts JR., Ernestina Schipani
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Abstract
DNA encoding a parathyroid hormone receptor; production and isolation of recombinant and synthetic parathyroid hormone receptor polypeptides and fragments; antibodies to parathyroid hormone receptors and receptor fragments; methods for screening candidate compounds for antagonistic or agonistic effects on parathyroid hormone receptor action; and diagnostic and therapeutic methods of these compounds are disclosed.
Claims39
Isolated DNA comprising a DNA sequence encoding a cell receptor of a vertebrate animal, said receptor having an amino acid sequence with at least 30% identity to the amino acid sequence shown in FIG. 3.
A purified preparation of a vector, said vector comprising a DNA sequence encoding a parathyroid hormone receptor.
Isolated DNA comprising a DNA sequence encoding a polypeptide capable of binding parathyroid hormone or parathyroid-hormone-related protein.
A method for producing a polypeptide, said method comprising:
- providing a ceil comprising isolated DNA encoding a parathyroid hormone receptor or a fragment thereof;
- and
- culturing said cell under conditions permitting expression of a polypeptide from said DNA.
A single-stranded DNA comprising a portion of a parathyroid hormone receptor gene, said portion being at least 18 nucleotides long.
A single-stranded DNA comprising a portion of a parathyroid hormone receptor cDNA, said portion being at least 18 nucleotides long.
Parathyroid hormone receptor produced by expression of a recombinant DNA molecule encoding a parathyroid hormone receptor.
A polypeptide comprising at least six amino acids and less than the complete amino acid sequence of a parathyroid hormone receptor, said polypeptide capable of binding parathyroid hormone or parathyroid hormone-related protein.
A therapeutic composition comprising, in a pharmaceutically-acceptable carrier, (a) a parathyroid hormone receptor or (b) a polypeptide comprising a fragment of said receptor.
An antibody capable of forming an immune complex with a parathyroid hormone receptor.
A compound capable of inhibiting the binding of parathyroid hormone or parathyroid hormone-related protein to a parathyroid receptor on the surface of a cell.
A transgenic non-human vertebrate animal bearing a transgene comprising a DNA sequence encoding parathyroid hormone receptor or a fragment thereof.
Dependencies are inferred from the claim text.