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US 2003/0153026 A1Published application
Entrapped binding protein as biosensors
OwnerJavier Alarcon, Christopher J. Knors +1inventors
InventorsJavier Alarcon, Christopher J. Knors, J. Bruce Pitner
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Abstract
The invention is directed to entrapped mutated binding proteins, mutated binding proteins containing reporter groups, compositions of mutated binding proteins containing reporter groups in analyte permeable matrixes, and their use as analyte biosensors both in vitro and in vivo
Claims50
A glucose biosensor for in vivo or in vitro use comprising:
- a) at least one mutated binding protein and at least one reporter group attached thereto such that said reporter group provides detectable and reversible signal when said mutated binding protein is exposed to varying glucose concentrations;
- and
- b) an analyte permeable matrix entrapping or encapsulating said at least one mutated binding protein.
A method for glucose detection comprising:
- a) providing at least one mutated glucose/galactose binding protein and at least one reporter group attached thereto;
- c) entrapping or encapsulating said at least one mutated glucose/galactose binding protein in an analyte permeable matrix;
- d) exposing said mutated glucose/galactose binding protein to varying glucose concentrations;
- and
- e) detecting signal from said reporter group
2 more elements
A composition comprising a mitixure of:
- a) a mutated glucose/galactose binding protein having at least one amino acid substitution selected from the group consisting of a cysteine at position 11, a cysteine at position 14, a cysteine at position 19, a cysteine at position 43, a cysteine at position 74, a cysteine at position 107, a cysteine at position 110, a cysteine at position 1 12, a cysteine at position 113, a cysteine at position 137, a cysteine at position 149, a cysteine at position 213, a cysteine at position 216, a cysteine at position 238, a cysteine at position 287, and a cysteine at position 292;
- and
- b) an analyte permeable matrix.
Dependencies are inferred from the claim text.