Compositions and methods for detecting protein modification and enzymatic activity

A kit for detecting protein modification, which kit comprises:

• a) a capture molecule immobilized on a solid support, said capture molecule is capable of specifically binding to a target protein;
• and
• b) means for assessing modification status and/or identity of said target protein.

An array of protein capture molecules, which array comprises:

• a) a solid support;
• and
• b) a plurality of capture molecules immobilized on said solid support, wherein each of said molecules is capable of specifically binding to both a modified and an unmodified form of a member protein of a plurality of target proteins.

An array of protein capture molecules, which array comprises:

• a) a solid support;
• and
• b) a plurality of capture molecules immobilized on said solid support, wherein each of said molecules is capable of specifically binding to an epitope generated by a specific modification moiety of a modified protein.

An array of enzyme substrates, which array comprises:

• a) a solid support;
• and
• b) a plurality of substrates immobilized on said solid support, wherein each of said substrates is a substrate of a member enzyme of a group of structurally and/or functionally related enzymes.

A method for detecting enzymatic activity in a sample, which method comprises:

• a) contacting a sample containing or suspected of containing a group of structurally and/or functionally related target enzymes with a plurality of substrates immobilized on a solid support, wherein each of said substrates is a substrate of a member enzyme of said group of target enzymes under conditions suitable for said target enzymes to catalyze enzymatic reactions involving said immobilized substrates;
• and
• b) assessing enzymatic activities of said target enzymes.