Biosensors, method for obtaining the same and uses thereof
AssigneesInstitut Pasteur, Centre National de la Recherche Scientifique (CNRS)
InventorsMartial Renard, Laurent Belkadi, Patrick England, Hugues Bedouelle
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Abstract
The invention concerns biosensors, the method for obtaining them and their uses in particular for detecting, assaying or locating, in direct immunofluorescence, a ligand such as an antigen or a hapten, in a heterogeneous population. Said biosensors consist of (i) at least a protein-type receptor fragment, capable of binding with a suitable ligand via an active site and in which fragment, at least one of its amino acid residues, located in the proximity of said active site is naturally present in the form of a Cys residue or is substituted in Cys residue, and (ii) a fluorophore coupled with said Cys residue(s).
Claims32
A biosensor, characterized in that it consists of (i) at least one fragment of a receptor which is protein in nature, capable of binding to a suitable ligand via an active site, and in which fragment at least one of its amino acid residues located in the proximity of said active site is naturally present in the form of a Cys residue, or is substituted with a Cys residue, and (ii) a fluorophore coupled to said Cys residue(s).
The plasmid pMR1, deposited with the Collection Nationale de Culture de Microorganismes (CNCM), under the number I-2386, dated Feb. 29, 2000.
The plasmid pMR1 (VL-S93C), deposited with the Collection Nationale de Culture de Microorganismes (CNCM), under the number I-2387, dated Feb. 29, 2000.
Dependencies are inferred from the claim text.