Methods and reagents for conducting multiplexed assays of multiple analytes

A method for assaying one or more target analytes in a sample, wherein said method comprises:

• (A) providing, for at least one target analyte to be assayed, a binding ligand of said target analyte, said binding ligand being bound to a solid support;
• wherein said support is porous and wherein binding ligand is bound to said support within the pores of said support and said pores sterically interfere with the ability of said binding ligand to bind to said target analyte and wherein the ability of said binding ligand to bind to said target analyte is hindered by a steric interference that does not hinder the binding of all other target analyte(s) to all other binding ligand(s);
• (B) determining, for such target analyte(s), the presence, absence, activity or concentration of said target analyte(s), by determining the extent of binding between said target analyte and said solid-support-bound binding ligand of said target analyte.

A method for assaying one or more target analytes in a sample, wherein said method comprises:

• (A) providing, for at least one target analyte to be assayed, a binding ligand of said target analyte, said binding ligand being bound to a solid support;
• wherein said support comprises bound interfering molecules that sterically interfere with the ability of said binding ligand to bind to said target analyte but does not hinder the binding of all other target analyte(s) to all other binding ligand(s);
• (B) determining, for such target analyte(s), the presence, absence, activity or concentration of said target analyte(s), by determining the extent of binding between said target analyte and said solid-support-bound binding ligand of said target analyte.

A method for assaying one or more target analytes in a sample, wherein said method comprises:

• (A) providing, for at least one target analyte to be assayed, a binding ligand of said target analyte, said binding ligand being bound to a solid support;
• wherein the ability of said binding ligand to bind to said target analyte is hindered by a chemical interference that does not hinder the binding of all other target analyte(s) to all other binding ligand(s);
• (B) determining, for such target analyte(s), the presence, absence, activity or concentration of said target analyte(s), by determining the extent of binding between said target analyte and said solid-support-bound binding ligand of said target analyte.

A method for assaying one or more target analytes in a sample, wherein said method comprises:

• (A) providing, for at least one target analyte to be assayed, a binding ligand of said target analyte, said binding ligand being bound to a solid support;
• wherein said support comprises bound interfering molecules that chemically interfere with the ability of said binding ligand to bind to said target analyte but which do not hinder the binding of all other target analyte(s) to all other binding ligand(s);
• (B) determining, for such target analyte(s), the presence, absence, activity or concentration of said target analyte(s), by determining the extent of binding between said target analyte and said solid-support-bound binding ligand of said target analyte.

A composition for assaying a target analyte, which comprises a binding ligand of said target analyte bound to a solid support, wherein said support provides a steric interference that hinders the ability of said target analyte to bind to said bound binding ligand.

A composition for assaying a target analyte, which comprises a binding ligand of said target analyte bound to a solid support, wherein said support provides a chemical interference that hinders the ability of said target analyte to bind to said bound binding ligand.

A kit for assaying a target analyte, which comprises:

• (A) a first container containing a binding ligand of said target analyte bound to a solid support, wherein said support provides a steric or chemical interference that hinders the ability of said target analyte to bind to said bound binding ligand;
• and
• (B) a second container containing a detectably labeled binding ligand-binding molecule.