Patterned biological molecules on inner surface of enclosed tubes
OwnerReid N. Orth, Harold G. Craighead +1inventors
InventorsReid N. Orth, Harold G. Craighead, Stephen W. Turner
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Abstract
Biomolecular photo-based patterning methods utilize avidin-biotin technology to immobilize functional proteins on the inner surface of silica glass tubes in desired patterns. The methods are useful for nanofluidic affinity biosensor/chromatography systems and on silicon dioxide substrates for biosensor applications. The resulting patterns are optimized based on the application. A zebra shaped pattern is utilized for an affinity chromatography system.
Claims38
A method of creating a desired biosample affinity in a glass tube, the method comprising:
- applying a photo activatable biotin to the inside of the glass tube;
- exposing the photo activatable biotin to light through a mask having a desired pattern;
- and
- removing unreacted photo activatable biotin.
A method of creating affinity in capillary tubes, the method comprising:
- coating the enclosed structure in a silane solution;
- applying a photoactivatable Biotin material to the inside of a capillary tube;
- exposing the enclosed structure to UV light through a mask having a desired pattern;
- removing unreacted photoactivatable Biotin;
- and
- incubating the tube with avidin.
A method of creating biosample affinity in enclosed structures, the method comprising:
- coating the enclosed structure;
- applying a photo activatable material to the enclosed structure;
- exposing the enclosed structure to UV light through a mask having a desired pattern;
- and
- removing unreacted photo activatable material.
A method of creating a pattern having biosample affinity on a silicon substrate, the method comprising:
- applying a photo activatable biomolecule supported by the substrate;
- exposing the substrate to light through a mask having a desired pattern;
- and
- removing unreacted photo activatable biomolecule.
A method of creating a pattern having biosample affinity on a surface, the method comprising:
- applying a silane layer supported by the substrate;
- applying a photo activatable biomolecule supported by the silane layer;
- exposing the layers to light through a mask having a desired pattern;
- and
- removing unreacted photo activatable biomolecule.
A container comprising:
- an inner and outer surface;
- a silane layer supported by the inner surface of the tube;
- a patterned photoactivatable biotin layer supported by the silane layer;
- and
- an avidin layer bound to the biotin layer.
The container of claim 20 wherein the silane layer comprises 3-aminopropyltriethoxysilane and the biotin layer comprises N-hydroxysuccinimide ester of photoactivatable biotin.
A small fluidic system comprising:
- a substrate having structures for handling biosamples;
- and
- a tube supported by the substrate and coupled to the structures, the tube having patterned immobilized functional proteins on an inner surface.
A method of creating a desired biosample affinity in an enclosed channel, the method comprising:
- applying an energy activatable reagent to the inside of the channel;
- exposing the energy activatable reagent to energy through a mask having a desired pattern to modify binding properties of the energy activatable reagent;
- and
- removing unexposed energy activatable reagent.
Dependencies are inferred from the claim text.