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US 2003/0152997 A1Published application
Haemobartonella PCR methods and materials
OwnerWayne A. Jenseninventor
Life of this patent
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Abstract
In broad terms, the present invention includes materials and methods useful to distinguish between and among species of a genus. The present methods utilize the differences in PCR amplicon sizes to specifically identify a given species.
Claims14
A method to detect Haemobartonella species in a test sample, comprising:
- a.) conducting polymerase chain reaction using starting materials which comprise a test sample and at least one set of genus-specific primers;
- and
- b.) detecting Haemobartonella species in the test sample in the event a Haemobartonella-sized amplicon is present.
A method to detect Haemobartonella-caused disease in a mammal, comprising:
- a.) conducting polymerase chain reaction using starting materials which comprise a test sample and at least one set of genus-specific primers;
- and
- b.) detecting Haemobartonella-caused disease in the test sample in the event a Haemobartonella-sized amplicon is present.
A method to detect anemia in a mammal, comprising:
- a.) conducting polymerase chain reaction using starting materials which comprise a test sample and at least one set of genus-specific primers;
- and
- b.) detecting anemia in the test sample in the event an amplicon is present, which amplicon is the size appropriate for a species chosen from the group consisting of:
- Haemobartonella felis Ohio;
- and Haemobartonella felis California.
2 more elements
A kit useful for distinguishing between or among Haemobartonella species, comprising at least one set of genus-specific primers.
A composition of matter comprising a set of genus-specific primers.
Dependencies are inferred from the claim text.