Method for nucleotide sequencing
AssigneeAjinomoto Co., Inc.
InventorEiichiro Kimura
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Abstract
The present invention relates to a method for nucleotide sequencing in which a nucleotide sequence of a single nucleotide or plural nucleotides can be determined by assaying reactions in one reaction solution. The nucleotide sequence can be determined according to the present method by detecting a reaction product continuously generated in an extension reaction system following the extension reaction of polynucleotide chain and thereby determining the alignment in the sequentially extending polynucleotide.
Claims11
A method for nucleotide sequencing, comprising:
- continuously generating a reaction product in a nucleotide extension reaction of a polynucleotide chain;
- detecting the reaction product;
- and
- determining the alignment in the sequentially extending polynucleotide.
The method according to clam 4, wherein the fusion substance is a deoxyribonucleotide 5′ triphosphate ester or a ribonucleotide 5′ triphosphate ester.
Dependencies are inferred from the claim text.