Tissue analysis and kits therefor
AssigneeGenentech, Inc.
InventorsRobert L. Cohen, Mary Beth Gardiner, Mark X. Sliwkowski, Gregory T. Stelzer
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Abstract
This invention relates to methods of analyzing a tissue sample from a subject. In particular, the invention combines morphological staining and/or immunohistochemistry (IHC) with fluorescence in situ hybridization (FISH) within the same section of a tissue sample. The analysis can be automated or manual. The invention also relates to kits for use in the above methods.
Claims23
A method of correlating cellular morphology with the presence of a cellular target nucleic acid sequence in a section of a tissue sample comprising the following steps:
- (a) staining the section of tissue sample with a morphological stain;
- (b) determining cellular morphology in the section of tissue sample;
- (c) hybridizing a first fluorescently labeled nucleic acid probe to the target nucleic acid sequence in the same section of tissue sample;
- (d) detecting the presence of the first nucleic acid probe in the section of tissue sample;
- and
- (e) correlating step (b) with step (d).
A method of correlating the presence of a cellular target protein with the presence of a cellular target nucleic acid sequence in a section of a tissue sample comprising the following steps:
- (a) contacting the section of sample tissue with an antibody which specifically binds to the target protein;
- (b) determining binding of the antibody to the section of tissue sample;
- (c) hybridizing a fluorescently labeled nucleic acid probe to the target nucleic acid sequence in the same section of tissue sample;
- (d) detecting the presence of the labeled nucleic acid probe in the section of tissue sample;
- and
- (e) correlating step (b) with step (d).
A kit comprising:
- (a) a morphological stain;
- (b) a fluorescently labeled probe complementary to a genetic abnormality;
- and (c) instructions for applying the stain (a) and probe (b) to the same section of tissue sample.
A kit comprising:
- (a) a primary antibody which specifically binds a cellular target protein;
- (b) a fluorescently labeled probe complementary to a genetic abnormality;
- and (c) instructions for applying the antibody (a) and probe (b) to the same section of tissue sample.
Dependencies are inferred from the claim text.