Method for the manufacture of DNA

A method for manufacturing DNA comprising the steps of

• a) preparing n single stranded base-DNA-oligonucleotides which form immediately consecutive parts of the nucleotide sequence of the DNA being manufactured, in which
• i) the second to the n-th base-DNA-oligonucleotide is phosphorylated at the 5′end and
• ii) n is at least 2;
• b) preparing at least (n−1) single stranded joint-DNA-oligonucleotides, applicable to which joint-DNA-oligonucleotide, the 3′terminal region of a joint-DNA-oligonucleotide is at least partially complementary to the 3′-terminal region of a base-DNA-oligonucleotide, and the 5′-terminal region of the same joint-DNA-oligonucleotide is at least partially complementary to the 5′-terminal region of the immediately following base-DNA-oligonucleotide, so that when a joint-DNA-oligonucleotide is hybridized with 2 immediately consecutive base-DNA-oligonucleotides a double-stranded DNA-hybrid is formed in the region of the joint-DNA-oligonucleotide;
• c) contacting the base-DNA-oligonucleotides with the joint-DNA-oligonucleotides;
• d) subjecting the product DNA-hybrid from step c) to a ligation reaction;
• e) subjecting the reaction product from step d) to an exonuclease reaction, in which the DNA strand of the reaction product of step d) formed by ligated base-DNA-oligonucleotides includes at least two cap-structures.