US 2003/0152977 A1Published application

Novel human G-protein coupled receptor, HGPRBMY34, and variants and methods of use thereof

OwnerChandra S. Ramanathan, Shuba Gopal +2inventors

InventorsChandra S. Ramanathan, Shuba Gopal, Gabriel A. Mintier, John N. Feder

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Abstract

The present invention describes the novel human G-protein coupled receptor (GPCR) HGPRBMY34 and its encoding polynucleotide. Also described are expression vectors, host cells, antisense molecules, and antibodies associated with the HGPRBMY34 polynucleotide and/or polypeptide of this invention. In addition, methods for treating, diagnosing, preventing, and screening for disorders or diseases associated with abnormal biological activity of HGPRBMY34 are described, as are methods for screening for modulators, e.g., agonists or antagonists, of HGPRBMY34 activity and/or function.

Claims20

Dependent claims

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:
1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:
2 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1, having GPCR activity;
(f) an isolated polynucleotide comprising nucleotides 1050 to 2162 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 372 of SEQ ID NO:
2 minus the start codon;
(g) an isolated polynucleotide comprising nucleotides 1047 to 2162 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 372 of SEQ ID NO:
2 including the start codon;
(h) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
1;
(i) a polynucleotide fragment of SEQ ID NO:
3 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(j) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
4 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(k) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
4 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(l) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
4 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(m) a polynucleotide encoding a polypeptide of SEQ ID NO:
4 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3, having GPCR activity;
(n) a polynucleotide encoding a polypeptide of SEQ ID NO:
4, which is hybridizable to SEQ ID NO:
3, having GPCR activity;
(o) an isolated polynucleotide comprising nucleotides 4 to 1107 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 369 of SEQ ID NO:
4 minus the start codon;
(p) an isolated polynucleotide comprising nucleotides 1 to 1107 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 369 of SEQ ID NO:
4 including the start codon;
(q) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
3;
and
(r) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(l), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

55 more elements

An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:

(a) a polypeptide fragment of SEQ ID NO:
2 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(b) a polypeptide fragment of SEQ ID NO:
2 or the encoded sequence included in ATCC Deposit No:
XXXXX, having GPCR activity;
(c) a polypeptide domain of SEQ ID NO:
2 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(d) a polypeptide epitope of SEQ ID NO:
2 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(e) a full length protein of SEQ ID NO:
2 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(f) a polypeptide comprising amino acids 2 to 372 of SEQ ID NO:
2, wherein said amino acids 2 to 372 comprising a polypeptide of SEQ ID NO:
2 minus the start methionine;
(g) a polypeptide comprising amino acids 1 to 372 of SEQ ID NO:
2;
(h) a polypeptide fragment of SEQ ID NO:
4 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(i) a polypeptide fragment of SEQ ID NO:
4 or the encoded sequence included in ATCC Deposit No:
XXXXX, having GPCR activity;
(j) a polypeptide domain of SEQ ID NO:
4 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(k) a polypeptide epitope of SEQ ID NO:
4 or the encoded sequence included in ATCC Deposit No:
XXXXX;
(l) a full length protein of SEQ ID NO:
4 or the encoded sequence included in ATCC Deposit No:
XXXXX;a full length protein of SEQ ID NO:
4;
(m) a polypeptide comprising amino acids 2 to 369 of SEQ ID NO:
4, wherein said amino acids 2 to 369 comprising a polypeptide of SEQ ID NO:
4 minus the start methionine;
and
(n) a polypeptide comprising amino acids 1 to 369 of SEQ ID NO:
4.

39 more elements

Dependencies are inferred from the claim text.

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Abstract

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:
1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:
2 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
1, having GPCR activity;
(f) an isolated polynucleotide comprising nucleotides 1050 to 2162 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 372 of SEQ ID NO:
2 minus the start codon;
(g) an isolated polynucleotide comprising nucleotides 1047 to 2162 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 372 of SEQ ID NO:
2 including the start codon;
(h) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
1;
(i) a polynucleotide fragment of SEQ ID NO:
3 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(j) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
4 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(k) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
4 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(l) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
4 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:
3;
(m) a polynucleotide encoding a polypeptide of SEQ ID NO:
4 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3, having GPCR activity;
(n) a polynucleotide encoding a polypeptide of SEQ ID NO:
4, which is hybridizable to SEQ ID NO:
3, having GPCR activity;
(o) an isolated polynucleotide comprising nucleotides 4 to 1107 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 369 of SEQ ID NO:
4 minus the start codon;
(p) an isolated polynucleotide comprising nucleotides 1 to 1107 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 369 of SEQ ID NO:
4 including the start codon;
(q) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
3;
and
(r) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(l), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

55 more elements