Methods for selective targeting
AssigneeGenencor International, Inc.
InventorsGiselle G. Janssen, Christopher J. Murray, Deborah S. Winetzky
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Abstract
A selective targeting method is disclosed which comprises contacting a peptide library with an anti-target to allow the peptides to bind; separating non-binding peptides from the anti-target bound peptides, contacting the non-binding anti-target peptides with a target allowing the unbound peptides to bind with the target to form a target-bound peptide complex; separating the target-bound peptide complex from peptides which do not bind to the target, and identifying the target-bound peptides. In one embodiment the target is skin or hair. In another embodiment the anti-target is hair when the target is skin, and the anti-target is skin when the target is hair.
Claims12
A method for screening a peptide library comprising the steps of,
- (a) contacting the peptide library with an anti-target to allow the peptides to bind with said anti-target;
- (b) separating unbound peptides;
- (c) contacting the unbound peptides with a selected target to allow said unbound peptides to bind with the target to form a target-bound peptide complex;
- (d) separating said target-bound peptide complex from peptides which do not bind to said target;
- and
- (e) identifying the target-bound peptides on the target-bound peptide complex,
- wherein said anti-target is skin or hair and said target is skin when the anti-target is hair or said target is hair when the anti-target is skin.
A method for screening a peptide library comprising the steps of,
- (a) contacting the peptide library with a selected target and an anti-target essentially simultaneously to allow the peptides to bind with said target to form a target-bound peptide complex;
- (b) separating the target-bound peptide complex from the anti-target, anti-target bound peptides and free peptides;
- and
- (c) identifying the target-bound peptides on the target-bound peptide complex,
- wherein said anti-target is skin or hair and said target is skin when the anti-target is hair or said target is hair when the anti-target is skin.
A skin binding peptide comprising the amino acid sequence of SEQ ID NO. 5 or a skin binding peptide having at least 50% amino acid sequence identity thereto and a binding affinity to skin about the same or greater than the binding affinity of SEQ ID NO. 5 to skin.
A skin binding peptide consisting essentially of the amino acid sequence of SEQ ID NO. 5.
A hair binding peptide comprising the amino acid sequence of SEQ ID NO. 7 or a hair binding peptide having at least 50% amino acid sequence identity thereto and a binding affinity to hair about the same or greater than the binding affinity of SEQ ID NO. 7.
A hair binding peptide consisting essentially of the amino acid sequence of SEQ ID NO. 7.
A hair binding peptide consisting essentially of the amino acid sequence of SEQ ID NO. 6.
Dependencies are inferred from the claim text.