Loading patent…
AssigneeMonsanto Technology LLC
InventorsCaius M.T. Rommens, Kathleen M.M. Swords, Hua Yan, Bei Zhang
Estimating the term…
The invention describes a new method to isolate disease resistance genes in plants. The method teaches to transiently express in susceptible plants large numbers of R-gene homologs or non-host inducible genes isolated from non-host resistant plants. These plants can be screened for either disease resistance or ability to respond with a hypersensitive response to pathogen-elicitor subjection. The invention also reports several R-genes and non-host inducible genes that have been successfully isolated using the described method. These R-genes trigger a hypersensitive response in tobacco that is dependent on the presence of the ubiquitous P. infestans elicitor INF1. The presented R-genes are predicted to be both the first R-genes isolated that confer resistance against P. infestans and the first R-genes involved in non-host resistance.
A process for identifying a nucleic acid sequence encoding a protein conferring resistance against a plant pathogen or elicitor comprising the steps of:
A nucleic acid segment conferring non-host disease resistance to plants by responding to an avirulence gene in plant pathogens.
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:1-10 or the complement thereof or a sequence that hybridizes to SEQ ID NO:
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:21-30 or the complement thereof or a sequence that hybridizes to SEQ ID NO:21-30 under conditions of high stringency.
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:31-36 or the complement thereof or a sequence that hybridizes to SEQ ID NO:31-36 under conditions of high stringency.
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:57 or the complement thereof or a sequence that hybridizes to SEQ ID NO:57 under conditions of high stringency.
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:58 or the complement thereof or a sequence that hybridizes to SEQ ID NO:58 under conditions of high stringency.
A nucleic acid segment that comprises the nucleic acid sequences of SEQ ID NO:60 or the complement thereof or a sequence that hybridizes to SEQ ID NO:60 under conditions of high stringency.
A recombinant DNA expression system comprising an expression vector into which is inserted a heterologous DNA conferring non-host disease resistance to plants by responding to an avirulence gene in plant pathogens.
A cell transformed with a heterologous DNA conferring non-host disease resistance to plants by responding to an avirulence gene in plant pathogens.
A transgenic plant transformed with a nucleic acid segment conferring non-host disease resistance to plants by responding to an avirulence gene in plant pathogens.
Diagnostic kits containing nucleic acid segments SEQ ID NO:1-10 or SEQ ID NO:57 or SEQ ID NO:58 or SEQ ID NO:60.
Dependencies are inferred from the claim text.