Methods and compositions for detecting target sequences
AssigneeThird Wave Technologies, Inc.
InventorsVictor Lyamichev, Bruce P. Neri, Jeff Hall, Andrew A. Lukowiak
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Abstract
The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Claims53
A method for detecting a target sequence, comprising:
- a) providing:
- i) a sample containing synthetic DNA amplified from genomic DNA, said genomic DNA suspected of containing said target sequence;
- ii) oligonucleotides capable of forming an invasive cleavage structure with said synthetic DNA when said genomic DNA contains said target sequence;
- and
- iii) an agent for detecting the presence of an invasive cleavage structure;
- and
- b) exposing said sample to said oligonucleotides and said agent.
A nucleic acid treatment kit comprising oligonucleotides capable of forming an invasive cleavage structure with synthetic DNA amplified from genomic DNA suspected of containing a target sequence when said genomic DNA contains a target sequence.
A kit for detecting the presence of a synthetic DNA comprising a FEN-1 endonuclease and amplification primers.
Dependencies are inferred from the claim text.