Method of assaying anti-ena antibody and assay kit
OwnerAkihiro Murakami, Kazuo Kojimainventors
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Abstract
A method for measuring an anti-ENA antibody having a high correlation with the conventional DID method and having a high sensitivity is provided. A complex of an RNA which is substantially the same as an RNA constituting ENA (Extractible Nuclear Antigen) and a protein molecule which is substantially the same as a intracellular non-histone soluble protein constituting ENA is formed and the relevant complex and a specimen are reacted. The resultant reaction product is detected.
Claims29
A method for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA and a first intracellular non-histone soluble protein, comprising the steps of:
- forming a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein, and then reacting the complex and a specimen.
A method for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA, a first intracellular non-histone soluble protein and a second intracellular non-histone soluble protein and whose recognition site is located at anyone of the first RNA, the first intracellular non-histone soluble protein or a complex thereof, comprising the steps of:
- forming a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein, and then reacting the relevant complex and a specimen.
A method for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA and a first intracellular non-histone soluble protein, the method comprising the following steps a)-c):
- a) forming a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein;
- b) reacting a specimen with the complex;
- and
- c) detecting a reaction product generated by the step b).
A method for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA, a first intracellular non-histone soluble protein and a second intracellular non-histone soluble protein and whose recognition site is located at anyone of the first RNA, the first intracellular non-histone soluble protein with a cell or a complex thereof, the method comprising the following steps a)-c):
- a) forming a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein;
- b) reacting a specimen with the complex;
- and
- c) detecting a reaction product generated by the step b).
A complex used for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA and a first intracellular non-histone soluble protein, the complex comprising a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein.
A complex used for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA, a first intracellular non-histone soluble protein and a second intracellular non-histone soluble protein and whose recognition site is located at any one of the RNA, the first intracellular non-histone soluble protein or a complex thereof, comprising:
- a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein.
A kit for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA and a first intracellular non-histone soluble protein, comprising a solid-phased antigen which has solid-phased a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein on an insoluble support;
- an anti-human immune globulin antibody;
- and the standard specimen containing an anti-ENA antibody.
A kit for measuring an anti-ENA antibody which specifically recognizes an antigen containing a first RNA, a first intracellular non-histone soluble protein and a second intracellular non-histone soluble protein and whose recognition site is located at any one of the RNA, the first intracellular non-histone soluble protein or a complex thereof, comprising:
- a solid-phased antigen which has solid-phased a complex of a second RNA which is substantially the same as the first RNA and a first protein molecule which is substantially the same as the first intracellular non-histone soluble protein on an insoluble support;
- an anti-human immune globulin antibody;
- and the standard specimen containing an anti-ENA antibody.
A method for measuring an anti-U1RNP which specifically recognizes an U1RNP consisted of an U1snRNA and an RNP, comprising:
- the step of forming a complex of an RNA which is substantially the same as the U1snRNA and a protein molecule which is substantially the same as the RNP, and then reacting the relevant complex and a specimen.
A method for measuring an anti-U1RNP antibody which specifically recognizes an U1RNP consisted of an U1snRNA and an RNP, comprising the following steps A)-C):
- A) forming a complex of an RNA which is substantially the same as the U1snRNA and a protein molecule which is substantially the same as the RNP,
- B) reacting a specimen with the complex, and
- C) detecting a reaction product generated by the step B).
A complex comprising an RNA which is substantially the same as an U1snRNA and a protein molecule which is substantially the same as an RNP.
A kit for measuring an anti-U1RNP antibody, the kit comprising:
- a solid-phased antigen that has solid-phased a complex of an RNA which is substantially the same as an U1snRNA and a protein molecule which is substantially the same as an RNP on an insoluble support, an anti-human immune globulin antibody and the standard specimen containing an anti-U1RNP antibody.
Dependencies are inferred from the claim text.