Detection of nerve tissue damage
OwnerJack Priceinventor
Life of this patent
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Abstract
This invention relates to diagnostics in the fields of neurotoxicology and neuropathology and more particularly to the visualisation of areas of damage to nerve tissue. In particular, the present invention relates to the use SCIP as a marker of neurological damage.
Claims29
A method of detecting neurological damage comprising assaying for the expression of a SCIP gene in nerve cells and/or tissue.
A kit for detecting SCIP in nerve cells and/or tissue expression comprising a first antibody molecule having affinity for SCIP protein, a second labelled antibody molecule having affinity for the first antibody molecule, development reagents to develop a colour reaction when in combination with the label of the second antibody, appropriate buffer diluents and a counterstain to stain nerve cells and/or tissue and provide contrast to SCIP containing material labelled using the first and second antibody molecules.
A kit for detecting SCIP expression in nerve cells and/or tissue by in situ hybridisation (ISH), wherein the kit comprises a labelled nucleic acid probe encoding a sequence complimentary to SCIP mRNA, buffered solutions for preincubation and incubation steps, a labelled antibody molecule having affinity for the labelled nucleic acid probe, development reagents which develop a colour reaction on contact with the labelled antibody molecule, appropriate buffered diluents and a counterstain to stain nerve cells and/or tissue and provide contrast to SCIP containing material which is labelled using the labelled nucleic acid probe and antibody molecule.
A method for assaying neurotoxicity of a test compounds comprising contacting nerve cells and/or tissue with a test compound and assaying for SCIP expression in nerve cells and/or tissue.
Dependencies are inferred from the claim text.