Polynucleotide encoding a novel human potassium channel alpha-subunit, K+alphaM2
OwnerJohn N. Feder, Liana Lee +1inventors
InventorsJohn N. Feder, Liana Lee, Han Chang
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Abstract
The present invention provides novel polynucleotides encoding K+alphaM2 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel K+alphaM2 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.
Claims20
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:
- (a) a polynucleotide fragment of SEQ ID NO:1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
- ZZZZZ, which is hybridizable to SEQ ID NO:1;
- (b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
- ZZZZZ, which is hybridizable to SEQ ID NO:1;
- (c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
- ZZZZZ, which is hybridizable to SEQ ID NO:1;
- (d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
- ZZZZZ, which is hybridizable to SEQ ID NO:1;
- (e) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or the cDNA sequence included in ATCC Deposit No:
- ZZZZZ, which is hybridizable to SEQ ID NO:1, having potassium channel activity;
- (f) an isolated polynucleotide comprising nucleotides 4 to 1275 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 425 of SEQ ID NO:2 minus the start codon;
- (g) an isolated polynucleotide comprising nucleotides 1 to 1275 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 425 of SEQ ID NO:2 including the start codon;
- (h) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1;
- and
- (i) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(h), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) a polypeptide fragment of SEQ ID NO:2 or the encoded sequence included in ATCC Deposit No:
- ZZZZZ;
- (b) a polypeptide fragment of SEQ ID NO:2 or the encoded sequence included in ATCC Deposit No:
- ZZZZZ, having potassium channel activity;
- (c) a polypeptide domain of SEQ ID NO:2 or the encoded sequence included in ATCC Deposit No:
- ZZZZZ;
- (d) a polypeptide epitope of SEQ ID NO:2 or the encoded sequence included in ATCC Deposit No:
- ZZZZZ;
- (e) a full length protein of SEQ ID NO:2 or the encoded sequence included in ATCC Deposit No:
- ZZZZZ;
- (f) a polypeptide comprising amino acids 2 to 425 of SEQ ID NO:2, wherein said amino acids 2 to 425 comprising a polypeptide of SEQ ID NO:2 minus the start methionine;
- and
- (g) a polypeptide comprising amino acids 1 to 425 of SEQ ID NO:2.
An isolated nucleic acid molecule consisting of a polynucleotide having a nucleotide sequence selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide of SEQ ID NO:2;
- (b) an isolated polynucleotide consisting of nucleotides 4 to 1275 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 425 of SEQ ID NO:2 minus the start codon;
- (c) an isolated polynucleotide consisting of nucleotides 1 to 1275 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 425 of SEQ ID NO:2 including the start codon;
- (d) a polynucleotide encoding the K+alphaM2 polypeptide encoded by the cDNA clone contained in ATCC Deposit No. ZZZZZ;
- and
- (e) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1.
An isolated polypeptide consisting of an amino acid sequence selected from the group consisting of:
- (a) a polypeptide fragment of SEQ ID NO:2 having potassium channel activity;
- (b) a polypeptide domain of SEQ ID NO:2 having potassium channel activity;
- (c) a full length protein of SEQ ID NO:2;
- (d) a polypeptide corresponding to amino acids 2 to 425 of SEQ ID NO:2, wherein said amino acids 2 to 425 consisting of a polypeptide of SEQ ID NO:2 minus the start methionine;
- (e) a polypeptide corresponding to amino acids 1 to 425 of SEQ ID NO:2;
- and
- (f) a polypeptide encoded by the cDNA contained in ATCC Deposit No. ZZZZZ.
Dependencies are inferred from the claim text.