Compositions and methods for random nucleic acid molecules
AssigneeStratagene
InventorsHolly H. Hogrefe, Janice M. Cline
Life of this patent
Estimating the term…
Abstract
The invention relates to compositions and methods for nucleic acid PCR mutagenesis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.
Claims31
A composition for PCR mutagenesis comprising an archaeal exo− DNA polymerase which substantially lacks 3′ to 5′ exonuclease activity, and PCR enhancing factor.
A kit for PCR mutagenesis comprising an archaeal exo− DNA polymerase, PCR enhancing factor, and packaging means therefor.
A method of PCR amplification for mutagenesis comprising incubating a reaction mixture comprising a nucleic acid template, at least two PCR primers, an archaeal exo− DNA polymerase, and PCR enhancing factor under conditions which permit amplification of said nucleic acid template by said archaeal exo− DNA polymerase to produce a mutated amplified product.
Dependencies are inferred from the claim text.