Proteins, genes and their use for diagnosis and treatment of breast cancer
OwnerHerath Mudiyanselage Athula Chandrasiri Herathinventor
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Abstract
The present invention provides methods and compositions for screening, diagnosis and prognosis of breast cancer, for monitoring the effectiveness of breast cancer treatment, and for drug development. Breast Cancer-Associated Features (BFs), detectable by two-dimensional electrophoresis of serum are described. The invention further provides Breast Cancer-Associated Protein Isoforms (BPIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated BPIs, antibodies immunospecific for BPIs, and kits comprising the aforesaid.
Claims34
A method for screening, diagnosis or prognosis of breast cancer in a subject, for determining the stage or severity of breast cancer in a subject, for identifying a subject at risk of developing breast cancer, or for monitoring the effect of therapy administered to a subject having breast cancer, said method comprising:
- (a) analyzing a test sample of body fluid from the subject by two dimensional electrophoresis to generate a two-dimensional array of features, said array comprising at least one chosen Breast Cancer-Associated Feature (BF) selected from BF-1, BF-2, BF-3, BF-4, BF-5, BF-7, BF-8, BF-9, BF-10, BF-12, BF-13, BF-14, BF-15, BF-16, BF-17, BF-18, BF-19, BF-20, BF-22, BF-23, BF-26, BF-27, BF-28, BF-29, BF-30, BF-31, BF-32, BF-33, BF-34, BF-35, BF-36, BF-37, BF-38, BF-39, BF-40, and BF-41, whose relative abundance correlates with the presence, absence, stage or severity of breast cancer or predicts the onset or course of breast cancer;
- and
- (b) comparing the abundance of each chosen feature in the test sample with the abundance of that chosen feature in body fluid from one or more persons free from breast cancer, or with a previously determined reference range for that feature in subjects free from breast cancer, or with the abundance at least one Expression Reference Feature (ERF) in the test sample.
A method for screening, diagnosis or prognosis of breast cancer in a subject, for determining the stage or severity of breast cancer in a subject, for identifying a subject at risk of developing breast cancer, or for monitoring the effect of therapy administered to a subject having breast cancer, comprising quantitatively detecting, in a sample of serum or plasma from the subject, at least one of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-40, BPI-48, BPI-49, BPI-50, BPI-51, BPI-52, BPI-53, BPI-54, BPI-55, BPI-56.
A method for screening, diagnosis or prognosis of primary breast cancer in a subject, comprising quantitatively detecting, in a sample of serum from the subject, at least one of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-40, BPI-50, BPI-53, BPI-54, BPI-55.
A method for screening, diagnosis or prognosis of metastatic breast cancer in a subject, comprising quantitatively detecting, in a sample of serum from the subject, at least one of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-48, BPI-49, BPI-51, BPI-52, BPI-53, BPI-56.
A preparation comprising the isolated Breast Cancer-Associated Protein Isoform (BPI) BPI-49.
A preparation comprising an isolated human protein, wherein the protein comprising a peptide having the following sequence:
- AN or AGG.
An antibody capable of immunospecific binding to one of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-40, BPI-48, BPI-49, BPI-50, BPI-51, BPI-52, BPI-53, BPI-54, BPI-55, BPI-56.
A method of treating or preventing breast cancer, comprising administering to a subject in need of such treatment or prevention a therapeutically effective amount of a nucleic acid encoding one or more of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-40, BPI-48, BPI-49, BPI-50, BPI-51, BPI-52, BPI-53, BPI-54, BPI-55, or BPI-56.
A method of treating or preventing breast cancer, comprising administering to a subject in need of such treatment or prevention a therapeutically effective amount of a nucleic acid that inhibits the expression of one or more of the following Breast Cancer-Associated Protein Isoforms (BPIs):
- BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-40, BPI-48, BPI-49, BPI-50, BPI-51, BPI-52, BPI-53, BPI-54, BPI-55, BPI-56.
A method of screening for or identifying agents that interact with a BPI, a BPI fragment, or a BPI-related polypeptide, comprising:
- (a) contacting a BPI, a BPI fragment, or a BPI-related polypeptide with a candidate agent;
- and
- (b) determining whether the candidate agent interacts with the BPI, the BPI fragment, or the BPI-related polypeptide.
A method of screening for or identifying agents that modulate the expression or activity of a BPI or a BPI-related polypeptide, comprising:
- (a) contacting a first population of cells expressing a BPI or a BPI-related polypeptide with a candidate agent;
- (b) contacting a second population of cells expressing BPI or BPI-related polypeptide with a control agent;
- and
- (c) comparing the level of expression of BPI or BPI-related polypeptide or mRNA encoding BPI or BPI-related polypeptide in the first and second populations of cells, or comparing the level of induction of a cellular second messenger in the first and second populations of cells.
A method of screening for or identifying agents that modulate the expression or activity of a BPI or a BPI-related polypeptide, comprising:
- (a) administering a candidate agent to a first mammal or group of mammals;
- (b) administering a control agent to a second mammal or group of mammals;
- (c) comparing the level of expression of the BPI or the BPI-related polypeptide or of mRNA encoding the BPI or the BPI-related polypeptide in the first and second groups, or comparing the level of induction of a cellular second messenger in the first and second groups;
- and
- (d) optionally comparing the levels of expression of the BPI or the BPI-related polypeptide or of mRNA encoding the BPI or the BPI-related polypeptide in the first and second groups, or comparing the level of induction of a cellular second messenger in the first and second groups, to the level of the BPI or the BPI-related polypeptide or of mRNA encoding the BPI or the BPI-related polypeptide in normal control mammals, or comparing the level of induction of a cellular second messenger in normal control mammals.
A method of screening for or identifying agents that modulate the activity of a BPI or a BPI-related polypeptide, comprising
- (a) in a first aliquot, contacting a candidate agent with the BPI or the BPI-related polypeptide;
- and
- (b) comparing the activity of the BPI or the BPI-related polypeptide in the first aliquot after addition of the candidate agent with the activity of the BPI or the BPI-related polypeptide in a control aliquot, or with a previously determined reference range.
An isolated nucleic acid molecule that hybridizes to a nucleotide sequence encoding BPI-49 or its complements.
An isolated nucleic acid molecule that hybridizes to a nucleotide sequence encoding at least 10 consecutive amino acids of BPI-49 or its complements.
A method for screening, diagnosis or prognosis of breast cancer in a subject or for monitoring the effect of an anti-breast cancer drug or therapy administered to a subject, comprising:
- (a) contacting at least one oligonucleotide probe comprising 10 or more consecutive nucleotides complementary to a nucleotide sequence encoding a BPI chosen from BPI-1, BPI-5, BPI-6, BPI-9, BPI-10, BPI-11, BPI-12, BPI-13, BPI-14, BPI-19, BPI-20, BPI-21, BPI-23, BPI-24, BPI-25, BPI-27, BPI-28, BPI-29, BPI-31, BPI-32, BPI-33, BPI-34, BPI-37, BPI-40, BPI-48, BPI-49, BPI-50, BPI-51, BPI-52, BPI-53, BPI-54, BPI-55, BPI-56 with an RNA obtained from a biological sample from the subject or with cDNA copied from the RNA wherein said contacting occurs under conditions that permit hybridization of the probe to the nucleotide sequence if present;
- (b) detecting hybridization, if any, between the probe and the nucleotide sequence;
- and
- (c) comparing the hybridization, if any, detected in step (b) with the hybridization detected in a control sample, or with a previously determined reference range.
An isolated nucleic acid molecule that hybridizes under highly stringent conditions or moderately stringent conditions to the nucleic acid sequence GCNAAY or the nucleic acid sequence GCCAAC.
Dependencies are inferred from the claim text.