High performance nucleic acid hybridization device and process
AssigneeIndustrial Technology Research Institute
InventorsYao-Sung Chang, Yung-Chiang Chung, Ming-Zheng Shiu
Life of this patent
Estimating the term…
Abstract
The invention discloses a device for hybridization reaction between a target molecule in a fluid and a probe, which comprises a microfluidic channel comprising a first portion and a second portion following said first portion; wherein said first portion have an irregular cross section and said second portion has a probe, and a fluid driving element connected the ends of said channel with tubes, wherein said fluid element can move said target molecules back-and-forth for repeatedly passing through said second portion.
Claims15
A device for hybridization reaction between a target molecule in a fluid and a probe, which comprises:
- a microfluidic channel comprising a first portion and a second portion following said first portion, wherein said first portion has an irregular cross section and said second portion has a probe, and
- a fluid driving element connected the ends of said channel with tubes, wherein said fluid element can move said target molecules back-and-forth for repeatedly passing through said second portion.
A process for increasing hybridization reaction between a target molecule and a probe, comprising the following steps:
- (a) providing a microfluidic channel comprising a first portion and a second portion following said first portion, wherein said first portion has an irregular cross section and said second portion has a first probe and second or more probes wherein said first probe specific binds to said target molecule;
- (b) introducing a fluid containing said target molecule into the microfluidic channel of the device for hybridization reaction of the invention;
- (c) driving said fluid to flow back and forth so that said target molecule can repeatedly pass through said second portion, whereby said target molecules non-specific binding to the second or more probes are removed and the target molecules binding to first probe are retained.
Dependencies are inferred from the claim text.