Closed substrate platforms suitable for analysis of biomolecules
OwnerMogens Havsteen Jakobsen, Lars Kongsbakinventors
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Abstract
The invention relates to novel analysis slides or substrates with a closed configuration. The slides may be used for any application which normally utilizes a conventional microscope slide and can be used in conjunction with any type of equipment typically used to manipulate or evaluate a standard microscope slide. In particular, the invention provides closed slides for covalent immobilization of polypeptides and nucleic acids. Further provided are methods for carrying out biological assays using arrays of biomolecules immobilized on the slides of the invention. The arrays of the invention are comprised of a unique meandering or straight channel design as compared to the rectangular design of conventional arrays.
Claims102
A closed substrate platform comprising:
- a slide element enclosed within a container;
- wherein,
- the slide comprises a defined microfluidic analysis platform;
- wherein,
- the container comprises at least one inlet port for introduction of liquid into the container which delivers liquid to the area for sample analysis;
- wherein, the container comprises at least one vent for expulsion of air from the container;
- and,
- the container comprises an outlet port for removal of liquid from the area for sample analysis.
The closed substrate platform of 7, wherein the inlet chamber is connected to a U-shaped channel.
A closed substrate platform comprising:
- a slide element enclosed within a container, preferably a sealed container with an opening that can provide fluid communication to the system, and the slide element preferably being plastic,
- wherein the slide comprises a defined area for sample analysis,
- wherein the container comprises at least one inlet port for introduction of liquid into the sealed container which delivers liquid to the area for sample analysis preferably via a tube or channel,
- wherein the sample analysis area is of a meandering design,
- wherein the sealed container comprises at least one vent for expulsion of air from the sealed container,
- wherein the sealed container comprises an outlet port for removal of liquid from the area for sample analysis preferably via a tube, channel, or the like.
A closed substrate platform comprising:
- a slide element enclosed within a container, preferably a sealed container with an opening that can provide fluid communication to the system, and the slide element preferably being plastic,
- wherein the slide comprises a defined area for sample analysis,
- wherein the sample analysis area is of a meandering design,
- wherein the container comprises a first inlet port for introduction of a sample into the sealed container and which delivers the sample to the area for sample analysis preferably via a tube or channel,
- wherein the container comprises a first vent for expulsion of air from the sealed container due to introduction of sample through the first inlet port,
- wherein the sealed container comprises a second inlet port for introduction of a fluid such as a wash buffer into the container and which delivers the fluid (preferably wash buffer) to the area for sample analysis preferably via a tube or channel, and
- wherein the container comprises a second vent for expulsion of air from the container due to introduction of fluid through the second inlet port.
A closed substrate platform comprising:
- a slide element enclosed within a container, preferably a sealed container with an opening that can provide fluid communication to the system, and the slide element preferably being plastic,
- wherein the slide comprises a defmed area for sample analysis,
- wherein the container comprises at least one inlet port for introduction of liquid into the sealed container which delivers liquid to the area for sample analysis preferably via a tube or channel,
- wherein the sample analysis area is a straight channel,
- wherein the inlet port is fitted with a rubber or silicon-based adaptor for introduction of a sample into the sealed container;
- and,
- said adaptor is conically shaped to allow for introduction of the sample into the adaptor with a minimized risk of spillage or back flow,
- wherein the fluid travels from the adapter to the analysis area through a canal which is interrupted by a flow restrictor, afterwhich,
- the fluid travels from the analysis area through a canal and arrives at a buffer chamber which is connected to a short capillary canal that opens into a waste area,
- wherein, the waste area ends in a vent, shaped as a capillary chamber.
Dependencies are inferred from the claim text.