Full-length human cDNAs encoding potentially secreted proteins
AssigneeSerono Genetics Institute S.A.
InventorsJean-Baptiste Dumas Milne Edwards, Lydie Bougueleret, Severin Jobert
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Abstract
The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for screening compounds that may be used in the treatment of GENSET-related disorders.
Claims22
An isolated polynucleotide, said polynucleotide comprising a nucleic acid sequence encoding:
- i) a polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs:
- 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool;
- or
- ii) a biologically active fragment of said polypeptide.
An isolated polypeptide or biologically active fragment thereof, said polypeptide comprising an amino acid sequence having any one of the sequences shown as SEQ ID NOs:
- 242-482 or any one of the sequences of polypeptides encoded by the clone inserts of the deposited clone pool.
A method of determining whether a GENSET gene is expressed within a mammal, said method comprising the steps of:
- a) providing a biological sample from said mammal
- b) contacting said biological sample with either of:
- i) a polynucleotide that hybridizes under stringent conditions to the polynucleotide of claim 1;
- or
- ii) a polypeptide that specifically binds to the polypeptide of claim 19;
- and
- c) detecting the presence or absence of hybridization between said polynucleotide and an RNA species within said sample, or the presence or absence of binding of said polypeptide to a protein within said sample;
- wherein a detection of said hybridization or of said binding indicates that said GENSET gene is expressed within said mammal.
Dependencies are inferred from the claim text.