Brcai and hmlhi gene primer sequences and method for testing
OwnerJan Vijginventor
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Abstract
Primer sequences and materials are pre-prepared as test kits for enabling appropriate gene scanning patterns, preferably by two dimensional electrophoresis (TDGS), for use in detecting sequence variations and/or mutations in BRCA1 and h MLH1 genes
Claims4
A method of enabling BRCA1 and h MLHI gene testing for gene sequence variation and/or mutations, that comprises, preparing appropriate test kit primers and solvents suitable for amplifying by long distance PCR the entire gene-coding region of each of BRCA1 and MLHI genes, to be followed by multiplex short PCR using the long PCR products as templates;
- providing in such test kit appropriate buffer and gel and solvent materials for use in electrophoresis in orthogonal dimensions for producing spot patterns indicative of gene sequence variations and/or mutations.
The method of claims wherein, the kits provide the Primer Pairs A and B listed in the specification for Long Distance and Short PCR, respectively.
Dependencies are inferred from the claim text.