Multiple viral replicon culture systems
AssigneeApath, LLC
InventorsJulie Dyall, Charles P. Romano, Paul D. Olivo, Robert M. Roth
Life of this patent
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Abstract
Methods and compositions are provided for screening candidate antiviral agents using cells containing subgenomic viral replication systems such as replicons and minigenomes. The methods involve the simultaneous assay of more than one subgenomic viral replication system. Compositions useful for these methods are also provided.
Claims69
A method of screening a candidate antiviral agent for antiviral activity comprising
- (a) preparing a first cell culture comprising cells containing a first subgenomic viral replication system, and a second cell culture comprising cells containing a second subgenomic viral replication system;
- (b) adding the candidate antiviral agent to each cell culture;
- (c) incubating the cell cultures under conditions and for a time sufficient to detect an antiviral effect by the candidate antiviral agent on the subgenomic viral replication systems;
- and
- (d) determining the effect of the candidate antiviral agent on each viral replication system,
- wherein the first subgenomic viral replication system is genetically distinct from the second subgenomic viral replication system.
A method of screening a candidate antiviral agent for antiviral activity comprising
- (a) combining a first cell culture comprising cells containing a first subgenomic viral replication system and a second cell culture comprising cells containing a second subgenomic viral replication system to make a mixed cell culture;
- (b) adding the candidate antiviral agent to the mixed cell culture;
- (c) incubating the mixed cell culture under conditions and for a time sufficient to detect an antiviral effect by the candidate antiviral agent on the subgenomic viral replication systems;
- and
- (d) determining the effect of the candidate antiviral agent on each viral replication system,
- wherein the first subgenomic viral replication system is genetically distinct from the second subgenomic viral replication system.
A mixed cell culture comprising a first cell culture comprising cells containing a first subgenomic viral replication system and a second cell culture comprising cells containing a second subgenomic viral replication system.
Dependencies are inferred from the claim text.