Method for identification and quantification of kinase inhibitors
OwnerMatthias Stein-Gerlach, Helmut Mett +2inventors
InventorsMatthias Stein-Gerlach, Helmut Mett, Manfred Marschall, Thomas Stamminger
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Abstract
The present invention relates to a method for the identification of kinase inhibitors which is suitable for high-throughput screening. Moreover, the inhibitory effect of test substances can be quantified and a potential cytotoxicity of the respective inhibitors can be detected. The method is particularly suitable for the identification of inhibitors of viral kinases, e.g. herpes viral kinases.
Claims25
A method for the identification of kinase inhibitors comprising the steps:
- (a) providing a target cell comprising a nucleic acid encoding a kinase,
- (b) adding to the target cell a substrate wherein said substrate is capable of being phosphorylated by said kinase and wherein said phosphorylated substrate is deleterious for said target cell,
- (c) adding to the target cell at least one test compound and
- (d) determining, if said test compound is capable of at least partially inhibiting the deleterious effect of said phosphorylated substrate.
A method for the identification of kinase inhibitors comprising the steps:
- (a) providing a target cell comprising a nucleic acid encoding a kinase,
- (b) adding to the target cell a substrate wherein said substrate is capable of being phosphorylated by said kinase and wherein said phosphorylated substrate is deleterious for said target cell,
- (c) adding to the target cell at least one test compound and
- (d) determining, if said test compound is capable of at least partially inhibiting the phosphorylation of the substrate.
A reagent kit for the identification of kinase inhibitors comprising a cell containing a nucleic acid encoding a kinase and a substrate capable of being phosphorylated by said kinase and wherein said phosphorylated substrate is deleterious for said control cell.
Dependencies are inferred from the claim text.