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US 2003/0152654 A1Published application
Ginkgo biloba composition, method to prepare the same and uses thereof
AssigneeShanghai Xingling Sci. & Tech. Pharmaceutical Co., Ltd.
InventorsDe Long Xie, Ning Wang, Qi Gao, Guo An Zhang, Bao Ping Shao, Xiao Wu Jin, Xin Sheng Huang
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Abstract
This invention provides different compositions extracted from Ginkgo biloba leaves. Said compositions comprise new active components. This invention also provides a method of preparation of the compositions and individual components of said compositions. Finally, this invention provides various uses of this composition.
Claims64
A composition comprising:
- (a) about 44% to about 78% flavonoids;
- (b) about 2.5% to about 10% ginkgolides selected from ginkgolide A, B, C, J or a combination thereof:
- (c) about 2.5% to about 10% bilobalide;
- and
- (d) about 0.1 ppm to about 5 ppm of ginkgolic acids.
2 more elements
A composition comprising:
- (a) about 44% to about 78% flavonoids that include flavonols, flavanols and flavonol glycosides;
- (b) about 2.5% to about 10% ginkgolides selected from ginkgolide A, B, C, J or a combination thereof:
- (c) about 2.5% to about 10% bilobalide;
- and
- (d) about 0.1 ppm to about 5 ppm of ginkgolic acids.
2 more elements
A composition comprising:
- (a) about 44% to about 78% flavonoids with a content of about 20% to about 75% flavonol glycosides;
- (b) about 2.5% to about 10% ginkgolides selected from ginkgolide A, B, C, J or a combination thereof;
- (c) about 2.5% to about 10% bilobalide;
- and
- (d) about 0.1 ppm to about 5 ppm ginkgolic acids.
2 more elements
A composition comprising:
- (a) about 44% to about 78% flavonoids which comprises flavonol glycosides and flavonols, with a content ratio between flavonol glycosides and flavonols as 1-30:1;
- (b) about 2.5% to about 10% ginkgolides selected from ginkgolide A, B, C, J or a combination thereof;
- (c) about 2.5% to about 10% bilobalide;
- and
- (d) about 0.1 ppm and 5 ppm ginkgolic acids.
2 more elements
A composition comprising:
- (a) about 44 to about 78% flavonoids comprising flavonol glycosides;
- (b) about 5% to 20% of lactones, wherein the lactones comprising 2.5% to about 10% ginkgolides selected from ginkgolide A, B, C, J or mixtures thereof and about 2.5% to about 10% bilobalide;
- (c) the ratio of flavonol glycosides to lactones is about 3.5-4.5:1;
- and
- (d) about 0.1 ppm and 5 ppm ginkgolic acids.
2 more elements
A composition comprising:
- (a) not less than 44% flavonoids comprising flavonol glycosides;
- (b) not less than 6% lactones comprising ginkgolides selected from ginkgolide A, B, C, J or a combination thereof and bilobalide;
- and
- (c) about 0.1 ppm and 5 ppm ginkgolic acids.
1 more element
A method for obtaining a Ginkgo biloba composition comprising steps of:
- (a) obtaining dried Ginkgo biloba leaves;
- (b) breaking the leaves into small pieces;
- (c) putting the broken leaves through a process of reflux in a solution selected from water, alkanols with C1 to C3, acetone, and a combination thereof under conditions permitting the extraction of flavonoids and lactones to produce an extract and a residue;
- (d) separating the extract from the residue;
- (e) concentrating the separated extract to a density of about 1.2 to about 1.25 at 60° C.;
- (f) applying the concentrate from step (e) to at least two kinds of resin under conditions permitting binding of flavonoids and lactones;
- (g) eluting the bound flavonoids and lactones, thereby producing an extract containing flavonoids and lactones from Ginkgo biloba.
4 more elements
A method for identification and examination of the flavones in a Ginkgo biloba composition comprising steps of:
- (a) preparing the assay by dissolving the composition in methanol;
- (b) preparing the standard by putting standard Ginkgo biloba leaves and 60% aqueous alcohol through a process of reflux;
- after filtration, concentrate the filtrate to evaporate off alcohol;
- extract the concentrated aqueous solution with petroleum, ethyl acetate and n-butanol respectively;
- concentrate the n-butanol portion to dryness and dissolve it in methanol;
- (c) performing the assay according to the TLC method by spotting each of the above-mentioned solution on the same thin silicon plate, developing the plate with a mixture of ethyl acetate, formic acid, acetic acid and water;
- then the plate is removed, air-dried, sprayed with 1% aluminum chloride in ethanol solution and observed under ultra-violet light at 365 nm;
- in both test and control chromatograms, eight yellow spots occur at the identical locations.
5 more elements
A method for identification and examination of the terpene lactones in a Ginkgo biloba composition comprising steps of:
- (a) preparing the assay by putting the composition and ethyl acetate through a process of reflux;
- after filtration, concentrate the filtrate to dryness and dissolve it in methanol;
- (b) preparing the standard by dissolving each standard sample of ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J and bilobalide in methanol to make five standard solutions;
- (c) performing the assay according to the TLC method by pipetting each of the above-mentioned solutions on the same silica gel GF254 thin layer plate respectively, developing the plate with a mixture of ethyl acetate, toluene, acetone and cyclohexane;
- then the plate is removed, air-dried, heated and observed under 254 nm ultra-violet light;
- in both test and control chromatograms spots of the same color occur at the identical locations.
3 more elements
A method for identification and examination of the ginkgolic acids in a Ginkgo biloba composition comprising steps of:
- (a) preparing the assay by putting the composition and n-hexane through a process of reflux;
- after filtration, concentrate the filtrate to dryness and dissolve it in ethyl acetate;
- (b) preparing the standard by adding ethyl acetate to standard compounds of ginkgolic acids;
- (c) performing the assay according to the TLC method by pipetting each of the above mentioned solutions on the same thin-silicon-plate (GF254);
- then the plate is developed with a mixture of n-hexane, ethyl-acetate and acetic acid, removed, air-dried and observed under 315 nm and 368 nm ultra-violet;
- the absorbance of the sample should be less than that of the standard solution.
3 more elements
A method for determination of the total amount of the flavonoids in a Ginkgo biloba composition comprising steps of:
- (a) preparing the standard by dissolving dry rutin with 70% aqueous alcohol;
- (b) obtaining the standard curve by pipetting different amount of the standard solution to a container;
- to each container add water, buffer (pH=4.5) of acetic acid, sodium acetate, 0.1 M aluminum chloride and 70% aqueous alcohol;
- plot the standard curve by obtaining the absorbance of each sample at 270 nm;
- (c) performing the assay according to the spectrophotometric method by dissolving the composition with 70% aqueous alcohol;
- pipetting the solution into a container and prepare the sample solution by using the same method described above;
- according to the standard curve, the concentration of the sample could be obtained by detecting its absorbance at 270 nm;
- the total content of flavonoids, calculated on the anhydrous basis, by rutin, is in the range of 85%-115% of the labeled amount.
5 more elements
A method for determination of the amount of the flavonol glycosides in a Ginkgo biloba composition comprising steps of:
- (a) preparing the assay by dissolving the composition with methanol and 25% hydrochloride and putting it through a process of reflux;
- then it is removed, left to cool and transferred to a container;
- the boiling container is washed with methanol and the washing solutions are decanted to the container, diluted to volume with methanol;
- (b) preparing the standard by dissolving quercetin, kaempferol and isorhamnetin in the same container;
- that is then diluted with methanol;
- (c) performing the assay by calculating the area of relative peaks on the HPLC spectrum in order to determine the amount of quercetin, kaempferol and isorhamnetin;
- the total amount of flavonol glycosides=amount of quercetin×2.50+amount of kaempferol×2.63+amount of isorhamnetin×2.36.
4 more elements
A method for determination of the amount of the terpene lactones in a Ginkgo biloba composition comprising steps of:
- (a) preparing the assay by putting the composition and acetone through a process of reflux;
- after filtration, concentrate the filtrate and dissolve the residue in methyl acetate, followed by an extraction with water;
- the water layer is extracted with methyl acetate again;
- the two methyl acetate layers are combined, concentrated to dryness and dissolved in methanol;
- (b) preparing the standard by dissolving each ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J and bilobalide with methanol in the same container;
- (c) performing the assay according to the HPLC test method by injecting SP and AP respectively to the column and their chromatogram are taken;
- the amount of ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J and bilobalide is calculated by the method for external standard in individual monograph, then they are summed up to obtain the total amount of terpene lactones.
4 more elements
Dependencies are inferred from the claim text.