Purified cytokine inhibitory factor
AssigneeArkion Life Sciences, LLC
InventorsSubramanian Iyer, William L. Johnson, Lance Nguyen, Steven C. Ross, Ruye Xing
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Abstract
Disclosed is a composition comprising a substantially purified Cytokine Inhibitory Factor (CIF) having certain characteristics, including the ability to inhibit RNA transcription of the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-2 (IL-2). Also disclosed is a method for substantially purifying the Cytokine Inhibitory Factor and a method for modulating the immune system of an animal using such composition.
Claims34
A composition comprising a substantially purified Cytokine Inhibitory Factor, wherein the Cytokine Inhibitory Factor:
- a) has molecular weight less than 6,000 Da;
- b) is naturally present in a lipid fraction of egg yolk of avian eggs;
- c) has a maximum wavelength absorbance at 205 nm;
- d) is a non-proteinatious substance;
- e) has a melting point of between about 39° C. to 42° C.;
- and
- f) inhibits RNA transcription of tumor necrosis factor alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-2 (IL-2).
A purified Cytokine Inhibitory Factor produced by a process comprising:
- a) separating a water-insoluble fraction (WIF) from a water-soluble fraction (WSF) of an egg yolk;
- b) separating the water-insoluble fraction into a neutral lipid fraction and a polar lipid fraction;
- c) purifying the polar lipid fraction into Cytokine Inhibitory Factor fractions by high performance liquid chromatography.
A method for purifying a Cytokine Inhibitory Factor from an avian egg, the egg comprising a yolk and a white, the method comprising:
- a) separating the egg into a water-insoluble fraction (WIF) and a water-soluble fraction (WSF);
- b) extracting a polar lipid fraction from the water-insoluble fraction and;
- c) purifying the polar lipid fraction into Cytokine Inhibitory Factor fractions by high performance liquid chromatography.
A method of modulating the immune system of an animal, comprising administering to the animal a composition comprising a Cytokine Inhibitory Factor, wherein the Cytokine Inhibitory Factor:
- a) has molecular weight less than 6,000 Da;
- b) is naturally present in a lipid fraction of egg yolk of avian eggs;
- c) has a maximum wavelength absorbance at 205 nm;
- d) is a non-proteinatious substance;
- e) has a melting point of between about 39° C. to 42° C.;
- and
- f) inhibits RNA transcription of tumor necrosis factor alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-2 (IL-2).
Dependencies are inferred from the claim text.