Mosaic infectious bursal disease virus vaccines
AssigneeID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V.
InventorsHendrik Johannis Boot, Anna Agnes, H., M. Ter Huurne, Bernardus Petrus, H. Peeters
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Abstract
The invention relates to Infectious Bursal Disease Virus (“IBDV”) and vaccines therefor. Provided are infectious recombinant Infectious Bursal Disease Virus (“rIBDV”) essentially incapable of growing in a cell that is not derived from a bursa cell, or an infectious rIBDV having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (“vvIBDV”).
Claims30
An infectious recombinant Infectious Bursal Disease Virus (rIBDV) essentially incapable of growing in a non-bursa cell or cell derived from a non-bursa cell.
An infectious rIBDV having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (vvIBDV).
A method for obtaining an infectious recombinant Infectious Bursal Disease Virus (rIBDV) incapable of growing on a non-bursa cell derived cell, said method comprising:
- transfecting at least one first cell with a nucleic acid comprising a IBDV genome at least partly derived from IBDV,
- incubating said first cell in a culture medium,
- recovering rIBDV from said transfected first cell or said culture medium and
- propagating said recovered rIBDV in at least one second cell which is permissive for said rIBDV.
A method for obtaining an infectious recombinant Infectious Bursal Disease Virus (rIBDV) having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (vvIBDV), said method comprising:
- transfecting at least one first cell with a nucleic acid comprising a IBDV genome at least partly derived from a vvIBDV,
- incubating said first cell in a culture medium,
- recovering rIBDV from said transfected first cell or said culture medium and
- propagating said recovered rIBDV in at least one second cell permissive for said vvIBDV.
An infectious mosaic IBDV (mIBDV) comprising a rIBDV wherein at least one genome segment comprises nucleic acid derived from at least two different Birna virus isolates.
Dependencies are inferred from the claim text.