US 2003/0152591 A1Published application

Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent

AssigneeInnogenetics N.V.

InventorsErwin Sablon, Leen-Jan Van Doorn, Wim Quint

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Abstract

The present invention relates to genomic nucleotide sequences and amino acid sequences corresponding to the non-coding and coding region of a new type of HCV. The invention relates to new HCV types and subtypes sequences which are different from the known HCV types and subtypes sequences. Particularly, the present invention relates to said new HCV type sequences; a process for preparing them, and their use for diagnosis, prophylaxis and therapy. More particularly, the present invention provides new type-specific sequences of the 5′ NCR, Core, the E1 and the NS5 regions of the new HCV type. These new HCV sequences are useful to diagnose the presence of HCV type genotypes or serotypes in a biological sample. Moreover, the availability of these new type-specific sequences can increase the overall sensitivity of HCV detection and should also prove to be useful for prophylactic and therapeutic purposes.

Claims47

Dependent claims

An isolated HCV polynucleic acid of a clade 6 HCV virus of a genotype different from clade 6 HCV genotypes 6-9 and 11, said polynucleic acid characterized in that it is comprising a nucleic acid sequence chosen from any of:

a nucleic acid sequence defined by any of SEQ ID NOs:
1, 2, 4, 6, 8, 10, 12, 14, 36-47, or 49;
a 5′UTR nucleic acid sequence comprising, relative to the start codon of the HCV polyprotein coding sequence, an adenine nucleotide at position −159;
a Core nucleic acid sequence at least 85% identical to any of SEQ ID NOs:
39-41;
an E1 nucleic acid sequence at least 71% identical to SEQ ID NO:
42;
a Core/E1 nucleic acid sequence at least 78% identical to any of SEQ ID NOs:
10, 12, 14;
a Core/E1 nucleic acid sequence at least 84% identical to any of SEQ ID NOs:
36-38;
an NS5B nucleic acid sequence at least 75% identical to SEQ ID NO:
8;
a nucleic acid sequence encoding a HCV polyprotein fragment defined by any of SEQ ID NOs:
3, 5, 7, 9, 11, 13, 15, 50-61, or 63;
a nucleic acid sequence encoding a Core protein fragment at least 92% identical to any of SEQ ID NOs:
53-55;
a nucleic acid sequence encoding an E1 protein fragment at least 79% identical to SEQ ID NO:
56;
a nucleic acid sequence encoding a Core/E1 protein fragment at least 85% identical to any of SEQ ID NOs:
11, 13, 15;
a nucleic acid sequence encoding a Core/E1 protein fragment at least 91% identical to any of SEQ ID NOs:
50-52;
a nucleic acid sequence encoding an NS5B protein fragment at least 87% identical to SEQ ID NO:
9;
a fragment of any of these nucleic acid sequences which is unique to said HCV genotype;
or
the complement of any of these nucleic acid sequences or of a fragment of any of these nucleic acid sequences which is unique to said HCV genotype.

25 more elements

An isolated HCV polypeptide of a clade 6 HCV virus of a genotype different from clade 6 genotypes 6-9 and 11, said polypeptide characterized in that it is comprising an amino acid sequence chosen from any of:

an amino acid sequence defined by any of SEQ ID NOs:
3, 5, 7, 9, 11, 13, 15, 50-61, or 63;
a Core amino acid sequence at least 92% identical to any of SEQ ID NOs:
53-55;
an E1 amino acid sequence at least 79% identical to SEQ ID NO:
56;
a Core/E1 amino acid sequence at least 85% identical to any of SEQ ID NOs:
11, 13, 15;
a Core/E1 amino acid sequence at least 91% identical to any of SEQ ID NOs:
50-52;
an NS5B amino acid sequence at least 87% identical to SEQ ID NO:
9;
a Core amino acid sequence encoded by a nucleic acid sequence at least 85% identical to any of SEQ ID NOs:
39-41;
an E1 amino acid sequence encoded by a nucleic acid sequence at least 71% identical to SEQ ID NO:
42;
a Core/E1 amino acid sequence encoded by a nucleic acid sequence at least 78% identical to any of SEQ ID NOs:
10, 12, 14;
a Core/E1 amino acid sequence encoded by a nucleic acid sequence at least 84% identical to any of SEQ ID NOs:
36-38;
an NS5B amino acid sequence encoded by a nucleic acid sequence at least 75% identical to SEQ ID NO:
8;
or
a fragment of any of these amino acid sequences which is unique to said HCV genotype.

21 more elements

Dependencies are inferred from the claim text.