Methods and compositions for the use of stromal cells to support embryonic and adult stem cells
OwnerChristopher Luft, William O. Wilkison +3inventors
InventorsChristopher Luft, William O. Wilkison, Bentley Cheatham, Jeffrey M. Gimble, Yuan-Di C. Halvorsen
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Abstract
The invention provides cells, compositions and methods based on the use of stromal cells to support the proliferation of undifferentiated embryonic or adult stem cells in vitro. The stem cells produced in the method are useful in providing a source of uncommitted or differentiated and functional cells for research, transplantation and development of tissue engineered products for the treatment of human diseases and traumatic tissue injury repair in any tissue or organ site within the body.
Claims37
A composition comprising an isolated stromal cell capable of supporting the in vitro proliferation and maintenance of stem cells in combination with a stem cell.
A method for the growth and maintenance of cultured stem cells comprising:
- i) isolating tissue-derived stromal cells;
- and
- ii) culturing the stromal cells in culture media with stem cells.
The method of claim 26, wherein the growth factors, cytokines, and chemokines are selected from the group consisting of:
- leukemia inhibitory factor, IL-1 through IL-13, IL-15 through IL-17, IL-19 through IL-22, granulocyte macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), macrophage colony stimulating factor (M-CSF), erythropoietin (Epo), thrombopoietin (Tpo), Flt3-ligand, B cell activating factor, artemin, bone morphogenic protein factors, epidermal growth factor (EGF), glial derived neurotrophic factor, lymphotactin, macrophage inflammatory proteins, myostatin, neurturin, nerve growth factors, platelet derived growth factors, placental growth factor, pleiotrophin, stem cell factor, stem cell growth factors, transforming growth factors, tumor necrosis factors, Vascular Endothelial Cell Growth Factors, and fibroblast growth factors, FGF-acidic and basic fibroblast growth factor.
Dependencies are inferred from the claim text.