Methods and apparatus for cell based screening
OwnerMichael A. Palladino JR., Scott M. Glaserinventors
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Abstract
Methods, kits and systems for identifying at least one compound, from a set of compounds, that modulates the growth or biological activity of a cell. Also high-throughput, tumor cell-based screening assay methods for identifying one or more cell-growth modulating compounds from among a library of compounds.
Claims44
A method of identifying at least one compound, from a set of compounds, that modulates the growth or biological activity of a cell, comprising the steps of:
- (a) contacting a cell to an agent that increases the sensitivity of the cell to cell death by at least three-fold, thereby yielding a sensitized or more differentiated cell;
- (b) contacting the sensitized cell to at least one compound from the set of compounds;
- and
- (c) identifying at least one compound that modulates the growth of the cell by detecting an indicia of cellular growth of the sensitized cell.
In a high-throughput, tumor cell-based screening assay method for identifying one or more cell-growth or differentiation modulating compounds from among a library of compounds, the improvement comprising the step of contacting the tumor cells to an agent that increases the sensitivity of the tumor cell to cell death.
A kit for identifying at least one compound, from a set of compounds, that modulates the growth or biological activity of a cell, comprising a container for contacting a compound or set of compounds with a sensitized cell and at least one additional component selected from the group consisting of a sensitizing agent, a cell, a reagent for detecting an indicia of cellular growth and/or biological or cellular activity, and a growth media.
Dependencies are inferred from the claim text.