Method and composition for hyperthermally treating cells
OwnerGholam A. Peymaninventor
Life of this patent
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Abstract
A method for the hyperthermia treatment of tissue in a target site applies a heat source to kill cells without protein denaturization. The method introduces an encapsulated dye that is released at a selected temperature in the target site to indicate that a threshold temperature has been reached to hyperthermally treat the tissue. In one embodiment, the composition releases the dye at a temperature of 42° C. to 50° C., and preferably about 45° C. to 49° C. The composition which can be a liposome composition encapsulating the dye can be introduced to the bloodstream of the patient to flow through the target site.
Claims34
A method of hyperthermally treating tissue in an animal, said method comprising the steps of:
- introducing a temperature indicating substance into the bloodstream of said animal to flow through a target site, said temperature indicating substance including a fluorescent dye encapsulated within a heat sensitive liposome, said fluorescent dye being releasable from said liposome at a temperature of at least 41° C., and
- applying a heat source to said target site and hyperthermally heating said target to at least 41° C. to release said dye and to hyperthermally treat said target site for a time sufficient to kill cells in said tissue, and fluorescing and visualizing said dye.
A method of detecting a threshold temperature and of hyperthermally treating tissue in an animal, said method comprising the steps of:
- introducing a first fluorescent dye encapsulated in a first heat sensitive liposome into the bloodstream of an animal in a location to flow through a target site in said animal, said first fluorescent dye being releasable from said first heat sensitive liposome at a temperature of at least 41° C.,
- heating said target site to a temperature to release said first fluorescent dye and fluorescing said first fluorescent dye to indicate and visualize a tissue temperature of at least 41° C., and continuing heating said target site at a temperature of at least 41° C. for a time sufficient to hyperthermally treat said tissue.
A method of hyperthermally treating tissue of an animal, said method comprising the steps of:
- introducing a temperature indicating substance into the bloodstream of said animal to flow through a target site, said temperature indicating substance including a first fluorescent dye encapsulated in a first temperature sensitive liposome, said first fluorescent dye being releasable from said first liposome by heating to a temperature of at least 42° C., and a second fluorescent dye encapsulated in a second temperature sensitive liposome, said second fluorescent dye being releasable from said second liposome by heating to a temperature of at least 50° C.,
- heating said target site and said first temperature sensitive liposome to a temperature of at least 42° C., and fluorescing said first fluorescent dye to indicate an effective temperature for hyperthermally treating said tissue without releasing said second fluorescent dye from said second liposomes.
Dependencies are inferred from the claim text.