Protein phosphatase stress-related polypeptides and methods of use in plants
AssigneeBASF Plant Science GmbH
InventorsHans J. Bohnert, Ruoying Chen, Manabu Ishitani, Nocha Van Thielen, Oswaldo da Costa e Silva
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Abstract
A transgenic plant transformed by a Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein expression or repression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated PPSRPs, and isolated nucleic acid coding PPSRPs, and vectors and host cells containing the latter.
Claims71
An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, or SEQ ID NO:31;
- b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32;
- and
- c) a polynucleotide complementary to a polynucleotide of any of a) through b) above.
An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32.
An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6.
An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32.
An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid selected from the group consisting of a polynucleotide sequence encoding a PP2A-1 and a PP-1 from Physcomitrella patens;
- a BnPP2A-1, a BnPP2A-2, and a BnPP2A-3 from Brassica napus;
- a GmPP2A-1, a GmPP2A-2, a GmPP2A-3, a GmPP2A-4, and a GmPP2A-5 from Glycine max;
- and an OsPP2A-1, an OsPP2A-2, an OsPP2A-3, an OsPP2A-4, and an OsPP2A-5 from Oryza sativa.
A method of modulating a plant's tolerance to an environmental stress comprising, modifying the expression of a Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid in the plant, wherein the nucleic acid is selected from the group consisting of:
- a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, or SEQ ID NO:31;
- b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:32;
- c) a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32;
- d) a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6;
- e) a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32;
- f) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) through e) above;
- and
- g) a polynucleotide complementary to a polynucleotide of any of a) through f) above.
A method of producing a transgenic plant containing a Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid wherein the plant has an increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising, transforming a plant cell with an expression vector comprising the nucleic acid and generating from the plant cell the transgenic plant, wherein the nucleic acid is selected from the group consisting of:
- a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, or SEQ ID NO:31;
- b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:32;
- c) a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32;
- d) a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6;
- e) a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32;
- f) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) through e) above;
- and
- g) a polynucleotide complementary to a polynucleotide of any of a) through f) above.
Dependencies are inferred from the claim text.