Transgenic plants with increased seed yield, biomass and harvest index

A method of increasing the biomass produced by a plant, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and,
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant produces increased biomass relative to a plant of the same genetic background in which said nucleic acid has not been introduced.

A method of increasing the Harvest Index of a plant, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and,
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant exhibits an increased Harvest Index relative to a plant of the same genetic background in which said nucleic acid has not been introduced.

A method of increasing the flag leaf weight of a monocotyledonous plant, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and,
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant exhibits increased flag leaf weight relative to a plant of the same genetic background in which said nucleic acid has not been introduced.

A method of increasing the number of seed heads produced by a monocotyledonous plant, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and, a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant produces an increased number of seed heads relative to a plant of the same genetic background in which said nucleic acid has not been introduced.

A method of increasing two or more traits of a dicotyledonous plant, wherein the traits are selected from the group consisting of the number of seeds, average seed weight, total seed weight, number of seed heads, Harvest Index and total plant weight, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and,
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant exhibits an increase in said two or more traits relative to a plant of the same genetic background in which said nucleic acid has not been introduced.

A method of increasing the yield of two or more traits of a monocotyledonous plant, wherein the traits are selected from the group consisting of the number of seeds, average seed weight, total seed weight, number of seed heads, flag leaf weight, Harvest Index and total plant weight, wherein the method comprises:

• a. introducing into a plant a nucleic acid, wherein the nucleic acid is selected from the group consisting of:
• a nucleic acid comprising SEQ ID NO:
• 1, or a fragment of SEQ ID NO:
• 1 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid which hybridizes with SEQ ID NO:
• 1 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 2, an allelic variant of SEQ ID NO:
• 2, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 2, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6;
• a nucleic acid comprising SEQ ID NO:
• 3, or a fragment of SEQ ID NO:
• 3 encoding a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• a nucleic acid which hybridizes with SEQ ID NO:
• 3 under high stringency conditions and encodes a polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and,
• a nucleic acid encoding a polypeptide comprising SEQ ID NO:
• 4, an allelic variant of SEQ ID NO:
• 4, a sequence which has at least 65% sequence identity to the relevant portion of SEQ ID NO:
• 4, or a fragment of said polypeptide that retains biological activity substantially the same as SH2−REV6-HS;
• and
• b. growing the plant produced in step a, wherein said plant exhibits an increase in said two or more traits relative to a plant of the same genetic background in which said nucleic acid has not been introduced.