DAS5, a P450 protein involved in the brassinosteroid biosynthesis pathway in plants
AssigneeThe Salk Institute for Biological Studies
InventorsJoanne Chory, Zhiyong Wang
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Abstract
The present invention provides a cytochrome P450-encoding gene, DAS5, as well as an altered gene, das5-D, which are involved in the synthesis of the plant steroid hormone brassinolide. The das5-D mutant has an insertion of a T-DNA containing transcriptional enhancers into its promoter region. Overexpression of the mutant thus produces large amounts of DAS5 transcript. The expression of this das5-D mutation or overexpression of the DAS5 gene in transgenic plants causes such plants to become significantly larger and more robust than their wild-type counterparts, thus increasing plant yields.
Claims28
A method of producing a genetically modified plant having increased size as compared to a wild-type plant, comprising:
- contacting a plant cell with at least one nucleic acid sequence encoding a DAS5 protein, said nucleic acid sequence operably associated with a promoter, to obtain a transformed plant cell;
- producing a plant from said transformed plant cell;
- and
- selecting a plant exhibiting said increased size.
A genetically modified plant exhibiting increased size in comparison to a wildtype plant, wherein said genetically modified plant comprises at least one exogenous nucleic acid sequence encoding a DAS5 polypeptide, wherein said polypeptide comprises an amino acid sequence with at least 80% sequence homology to SEQ ID NO:
- 1.
A genetically modified seed, wherein said seed produces a plant exhibiting increased size in comparison to a wildtype plant, wherein said genetically modified seed comprises at least one exogenous nucleic acid sequence encoding a DAS5 polypeptide, wherein said polypeptide comprises an amino acid sequence with at least 80% sequence homology to SEQ ID NO :
- 1.
A substantially purified DAS5 polypeptide having cytochrome P450 activity and functioning in the brassinolide biosynthetic pathway.
An antibody which binds to an isolated DAS5 polypeptide or antigenic fragments thereof.
An isolated DAS5 polynucleotide encoding the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.