US 2003/0150024 A1 · Plant MSH2 sequences and methods of use

Abstract

The invention relates to isolated nucleic acid molecules encoding MutS homologues (MSHs). Such MSH proteins are involved in DNA mismatch-repair processes in organisms. The invention provides isolated nucleic acid molecules comprising MSH2 nucleotide sequences which encode MSH2 proteins and MSH2 nucleotide sequences which encode dominant-negative MSH2 variants. Such MSH2 nucleotide sequences find use in altering mismatch repair, mutation rates and recombination frequencies in both eukaryotic and prokaryotic organisms. The invention also provides isolated nucleic acid molecules comprising MSH2 promoter nucleotide sequences. Such MSH2 promoter nucleotide sequences find use in regulating the expression of genes of interest in plants. Additionally provided are isolated proteins, transformed host cells, and transformed plants, tissues, cells and seeds thereof.

Claims37

Dependent claims

An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence set forth in SEQ ID NO:
2 or 4;
(b) the amino sequence comprising residues 1-266 of the amino acid sequence set forth in SEQ ID NO:
2 or 4;
(c) the amino acid sequence encoded by SEQ ID NO:
1 or 3;
(d) an amino acid sequence comprising at least 50 contiguous amino acids of at least one amino acid sequence selected from the group consisting of SEQ ID NOS:
2 and 4;
(e) an amino acid sequence comprising at least 85% identity to at least one amino acid sequence selected from the group consisting of SEQ ID NOS:
2 and 4;
and
(f) a fragment or variant of (a) that confers a dominant-negative phenotype in a host cell.

9 more elements

Dependencies are inferred from the claim text.

Plant MSH2 sequences and methods of use

Life of this patent

Plant MSH2 sequences and methods of use

Life of this patent

Abstract

Claims37