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AssigneeLarge Scale Biology Corporation
InventorsThomas H. Turpen, Ann Myers Turpen, Stephen J. Garger, Laurence K. Grill, Jonathan Donson, William O. Dawson, George L. Granthan
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The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular adivantage that it systemically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates to viruses containing the viral vectors which are infective, production cells which are capable of producing the viruses or parts thereof, a host infected by the viruses of the invention, the gene products produced by expression of the viral nucleic acids and a process for the production of a desired product by growing the infected hosts.
An RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the absence of a trans-acting replication element in said host cell.
A DNA transcription vector comprising cDNA having one strand complementary to an RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the absence of a trans-acting replication element in said host cell.
A method of modifying a host cell, genotypically or phenotypically, which method comprises introducing into the cell an RNA molecule comprising a cis-acting replication element derived from a (+) strand RNA plant virus capable of replication in a plant cell and further comprising an exogenous RNA segment capable of expressing its function in a host cell, said exogenous RNA segment being located in a region of said RNA molecule able to tolerate said segment without disrupting RNA replication of said RNA molecule in the absence of a transacting replication element in said host cell, whereby the exogenous RNA segment confers a detectable trait in the host cell, thereby modifying said host cell.
A capped RNA molecule capable of infecting a host plant cell, which capped RNA molecule comprises a cis-acting replication element derived from a (+) strand RNA plant virus, which capped molecule has no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, and which capped RNA molecule further comprises an exogenous RNA segment capable of expressing its function in a host cell in a region of said capped RNA molecule able to tolerate said segment without disrupting RNA replication of said capped RNA molecule, in the absence of a trans-acting replication element in said host cell.
A DNA transcription vector comprising cDNA having one strand complementary to a capped RNA molecule capable of infecting a host cell, which capped RNA molecule comprises a cis-acting replication element derived from a (+) strand RNA plant virus, which capped molecule has no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, and which capped RNA molecule further comprises an exogenous RNA segment capable of expressing its function in a host cell in a region of said capped RNA molecule able to tolerate said segment without disrupting RNA replication of said capped RNA molecule, in the absence of a trans-acting replication element in said host cell.
A method of modifying a host plant cell, genotypically or phenotypically, which method comprises introducing into the cell a capped RNA molecule capable of infecting said host cell, wherein the capped RNA molecule comprises a cis-acting replication element derived from a (+) strand RNA plant virus, wherein the capped RNA molecule has the same capped 5′ end as said virus such that there are no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, and wherein the capped RNA molecule further comprises an exogenous RNA segment in a region of said capped RNA molecule able to tolerate said segment without disrupting RNA replication of said capped RNA molecule in the absence of a trans-acting replication element in said host cell, whereby the exogenous RNA segment confers a detectable trait in the host cell, thereby modifying said host cell.
A capped RNA molecule capable of infecting a host plant cell, said capped RNA molecule having no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, said capped RNA molecule comprising:
A method of modifying a host plant cell, genotypically or phenotypically, which method comprises introducing to the cell a capped RNA molecule capable of infecting a host plant cell, said capped RNA molecule having no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, said capped RNA molecule comprising:
A DNA transcription vector comprising DNA having one strand complementary to a capped RNA molecule capable of infecting a host plant cell, said capped RNA molecule having no extraneous nonviral sequences between the cap site and the 5′ terminus of the viral sequence, said capped RNA molecule comprising:
Dependencies are inferred from the claim text.