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AssigneeMonsanto Technology LLC
InventorsSusan R. Norris, Kim Lincoln, Joshua C. Stein, Henry E. Valentin, Alison Van Eenennaam
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The present invention relates to genes associated with the tocopherol biosynthesis pathway. More particularly, the present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with genes that encode polypeptides that have methyltransferase activity. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express the aforementioned polypeptides. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.
A substantially purified nucleic acid molecule encoding a plant polypeptide having 2-methylphytylplastoquinol methyltransferase activity.
A substantially purified nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A substantially purified plant polypeptide molecule having 2-methylphytylplastoquinol methyltransferase activity.
A transformed plant comprising an introduced nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced nucleic acid molecule that encodes a polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced nucleic acid molecule that encodes a polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule that encodes a polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule that encodes a polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
A method for reducing, in a plant, expression of a gene encoding a plant polypeptide having 2-methylphytylplastoquinol methyltransferase activity, comprising:
A transformed plant comprising a nucleic acid molecule comprising an introduced promoter region which functions in plant cells to cause the production of an mRNA molecule, wherein said introduced promoter region is linked to a transcribed nucleic acid molecule having a transcribed strand and a non-transcribed strand, wherein said transcribed strand is complementary to a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A method of producing a plant having a seed with an increased γ-tocopherol level comprising:
A method of producing a plant having a seed with one or both of an increased γ-tocopherol level and increased γ-tocotrienol level comprising:
A method of producing a plant having a seed with one or both of an increased γ-tocopherol level and increased γ-tocotrienol level comprising:
A method of producing a plant having a seed with one or both of an increased γ-tocopherol level and increased γ-tocotrienol level comprising:
A method of producing a plant having a seed with one or both of an increased α-tocopherol level and increased α-tocotrienol level comprising:
A method of producing a plant having a seed with one or both of an increased α-tocopherol level and increased α-tocotrienol level comprising:
Seed derived from a transformed plant, wherein said transformed plant comprises an introduced nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
Seed derived from a transformed plant, wherein said transformed plant comprises an introduced first nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
Seed derived from a transformed plant, wherein said transformed plant comprises an introduced first nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule comprising a sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule that encodes a polypeptide molecule comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
A transformed plant comprising an introduced first nucleic acid molecule encoding a tMT2 enzyme and an introduced second nucleic acid molecule encoding a GMT enzyme.
A method of producing a plant having seed with an increased α-tocopherol level comprising:
A method of producing a plant having a seed with an increased total tocopherols level comprising:
Dependencies are inferred from the claim text.